微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2015年
3期
59-61,64
,共4页
丙型肝炎%抗黏病毒基因A%丙型肝炎病毒载量
丙型肝炎%抗黏病毒基因A%丙型肝炎病毒載量
병형간염%항점병독기인A%병형간염병독재량
Hepatitis C%Myxovirus resistance A%HCV RNA load
目的：：分析急性、慢性丙型肝炎患者抗黏病毒基因 A(MxA)mRNA 表达。方法：选择丙型肝炎病毒(HCV)感染者149例,其中,急性丙型肝炎(AHC)患者71例(AHC 组),慢性丙型肝炎(CHC)患者78例(CHC组)；另选体检健康人群69例为正常对照组。采用实时荧光定量 PCR 检测 MxA mRNA 表达水平及 HCV RNA载量,统计分析组间各指标的差异和相关性。结果：AHC 组和 CHC 组 MxA mRNA 相对表达量均明显高于正常对照组(P <0.01),且 AHC 组 MxA mRNA 的表达水平低于 CHC 组,差异有统计学意义(P <0.01)。AHC 组MxA mRNA 表达量与 HCV RNA 载量呈负相关(r =-0.865,P <0.001),CHC 组 MxA mRNA 表达量与 HCV RNA 载量无明显相关性(r=0.174,P >0.05)。结论：急性、慢性丙型肝炎患者 MxA mRNA 表达水平明显增高,可以作为 HCV 感染的辅助诊断指标。
目的：：分析急性、慢性丙型肝炎患者抗黏病毒基因 A(MxA)mRNA 錶達。方法：選擇丙型肝炎病毒(HCV)感染者149例,其中,急性丙型肝炎(AHC)患者71例(AHC 組),慢性丙型肝炎(CHC)患者78例(CHC組)；另選體檢健康人群69例為正常對照組。採用實時熒光定量 PCR 檢測 MxA mRNA 錶達水平及 HCV RNA載量,統計分析組間各指標的差異和相關性。結果：AHC 組和 CHC 組 MxA mRNA 相對錶達量均明顯高于正常對照組(P <0.01),且 AHC 組 MxA mRNA 的錶達水平低于 CHC 組,差異有統計學意義(P <0.01)。AHC 組MxA mRNA 錶達量與 HCV RNA 載量呈負相關(r =-0.865,P <0.001),CHC 組 MxA mRNA 錶達量與 HCV RNA 載量無明顯相關性(r=0.174,P >0.05)。結論：急性、慢性丙型肝炎患者 MxA mRNA 錶達水平明顯增高,可以作為 HCV 感染的輔助診斷指標。
목적：：분석급성、만성병형간염환자항점병독기인 A(MxA)mRNA 표체。방법：선택병형간염병독(HCV)감염자149례,기중,급성병형간염(AHC)환자71례(AHC 조),만성병형간염(CHC)환자78례(CHC조)；령선체검건강인군69례위정상대조조。채용실시형광정량 PCR 검측 MxA mRNA 표체수평급 HCV RNA재량,통계분석조간각지표적차이화상관성。결과：AHC 조화 CHC 조 MxA mRNA 상대표체량균명현고우정상대조조(P <0.01),차 AHC 조 MxA mRNA 적표체수평저우 CHC 조,차이유통계학의의(P <0.01)。AHC 조MxA mRNA 표체량여 HCV RNA 재량정부상관(r =-0.865,P <0.001),CHC 조 MxA mRNA 표체량여 HCV RNA 재량무명현상관성(r=0.174,P >0.05)。결론：급성、만성병형간염환자 MxA mRNA 표체수평명현증고,가이작위 HCV 감염적보조진단지표。
Objective:To explore the expression of myxovirus resistance A(MxA)in the patients infected with HCV.Method:Peripheral blood was collected from 149 patients with hepatitis C infection including 71 patients with acute hepatitis C infection(AHC)and 78 patients with chronic hepatitis C infection(CHC).In addition,69 healthy individuals as a control group.The expression of MxA mRNA and HCV RNA was detected by real-time quantitative PCR.Results:The expression of MxA mRNA in AHC and CHC group were significant higher than healthy group (P <0.01).The expression of MxA mRNA in AHC group was lower than CHC group(P <0.01).The expression of MxA mRNA was negative correlation with HCV RNA load in AHC group (r=-0.865,P <0.05)but no corre-lation with HCV RNA load in CHC group(r=0.1 74,P >0.05).Conclusion:The level of MxA gene expression of hepatitis C patients was significant higher than healthy person.MxA can be used as a diagnostic marker for HCV in-fection.
