CAJ | 학술논문

目的：拟寻求一种更好的大鼠体脑组织灌注方法以提高灌注固定效果。方法：健康雄性SD大鼠经10%水合氯醛进行麻醉，暴露心脏和主动脉弓，以小动脉夹夹闭胸主动脉，用动脉留置针在大鼠心尖波动最明显处进针，进入约0.5 cm时，边退出针芯，边将套管自左侧心室推送至升主动脉直至套管针尾部，并关闭套管针尾端的开关，防止血液外溢，连接输液管，剪破右心耳，依次从心脏灌注0.9%复方氯化钠注射液和4%多聚甲醛固定脑组织。结果：脑组织灌注所需的灌注液减少，灌注插管时间及总灌注时间均缩短，且灌注后脑组织更硬、色泽更白。结论：采用此种灌注方法后，其灌注固定效果明显提高，且操作过程简单易行，值得推广。
목적：의심구일충경호적대서체뇌조직관주방법이제고관주고정효과。방법：건강웅성SD대서경10%수합록철진행마취，폭로심장화주동맥궁，이소동맥협협폐흉주동맥，용동맥류치침재대서심첨파동최명현처진침，진입약0.5 cm시，변퇴출침심，변장투관자좌측심실추송지승주동맥직지투관침미부，병관폐투관침미단적개관，방지혈액외일，련접수액관，전파우심이，의차종심장관주0.9%복방록화납주사액화4%다취갑철고정뇌조직。결과：뇌조직관주소수적관주액감소，관주삽관시간급총관주시간균축단，차관주후뇌조직경경、색택경백。결론：채용차충관주방법후，기관주고정효과명현제고，차조작과정간단역행，치득추엄。
Objective:To seek a better method for in vivo perfusion of rat brain tissues and improve the efficiency of perfusion fixation.Method:The healthy male SD rat was anesthetized by 10% chloral hydrate,exposed the heart and aortic arch,clipping the thoracic aorta with small arteries clip,with artery remaining needle in the tip of the heart of the rat fluctuation most obviously needled into about 0.5 cm,exited the needle core,at the same time pushed the cannula from the left ventricle to the aorta until the trocar tail,and closed the cannula needle end of the switch to prevent blood spills,then connected with the infusion pipe and cuted the right auricle,fixation brain tissue from the heart perfused sequentially with 0.9%compound sodium chloride injection and 4% paraformaldehyde.Result:The required perfusion fluid of Cerebral tissue perfusion was reduced, perfusion intubation time and total perfusion time was shortened,and the brain tissue after the infusion was harder,the color was more white.Conclusion:By this method of perfusion,the perfusion fixation effect is obviously improved and the operation process is simple and easy to operate,which is worthy of promotion.