中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
24期
1-4
,共4页
王宗站%杨金霞%周宓%邢晓波
王宗站%楊金霞%週宓%邢曉波
왕종참%양금하%주복%형효파
紫杉醇纳米粒子%紫杉醇%Ca2+%多核细胞
紫杉醇納米粒子%紫杉醇%Ca2+%多覈細胞
자삼순납미입자%자삼순%Ca2+%다핵세포
Paclitaxel lipid nanoparticles%Paclitaxel%Ca2+%Multinucleated cells
目的：比较紫杉醇脂质纳米粒子（TAX-NLC）对KB、SKOV3细胞的抑制作用。方法：激光共聚焦显微镜观察TAX-NLC作用于KB、SKOV3细胞对Ca2+的影响；MTT法测定TAX-NLC作用于KB、SKOV3细胞抑制率；激光共聚焦显微镜观察TAX-NLC作用于KB、SKOV3细胞形态学的变化。结果：TAX-NLC作用后KB细胞Ca2+浓度高于SKOV3细胞；TAX-NLC作用后KB细胞的抑制率高于SKOV3细胞；TAX-NLC作用KB细胞产生多核细胞高于SKOV3。结论：TAX-NLC对KB细胞更敏感，Ca2+浓度、抑制率、多核细胞比例密切相关联。
目的：比較紫杉醇脂質納米粒子（TAX-NLC）對KB、SKOV3細胞的抑製作用。方法：激光共聚焦顯微鏡觀察TAX-NLC作用于KB、SKOV3細胞對Ca2+的影響；MTT法測定TAX-NLC作用于KB、SKOV3細胞抑製率；激光共聚焦顯微鏡觀察TAX-NLC作用于KB、SKOV3細胞形態學的變化。結果：TAX-NLC作用後KB細胞Ca2+濃度高于SKOV3細胞；TAX-NLC作用後KB細胞的抑製率高于SKOV3細胞；TAX-NLC作用KB細胞產生多覈細胞高于SKOV3。結論：TAX-NLC對KB細胞更敏感，Ca2+濃度、抑製率、多覈細胞比例密切相關聯。
목적：비교자삼순지질납미입자（TAX-NLC）대KB、SKOV3세포적억제작용。방법：격광공취초현미경관찰TAX-NLC작용우KB、SKOV3세포대Ca2+적영향；MTT법측정TAX-NLC작용우KB、SKOV3세포억제솔；격광공취초현미경관찰TAX-NLC작용우KB、SKOV3세포형태학적변화。결과：TAX-NLC작용후KB세포Ca2+농도고우SKOV3세포；TAX-NLC작용후KB세포적억제솔고우SKOV3세포；TAX-NLC작용KB세포산생다핵세포고우SKOV3。결론：TAX-NLC대KB세포경민감，Ca2+농도、억제솔、다핵세포비례밀절상관련。
Objective:To compare the inhibitory effect of paclitaxel lipid nanoparticles(TAX-NLC)on KB,SKOV3 cells. Method:The affection of TAX-NLC on Ca2+in KB,SKOV3 cells by using Laser confocal microscope were observed.MTT method was used to determine the inhibition rate of TAX-NLC action in KB,SKOV3 cell.The cell morphological changes of TAX-NLC role in KB,SKOV3 cells were observed by Laser confocal microscope.Result:After the TAX-NLC acting on KB,SKOV3 cells,the concentration of Ca2+of KB cells was higher than that of SKOV3 cells.And the inhibition rate of KB cell was higher than that of SKOV3 cells,the production of KB multinucleated cells was more than that of SKOV3 cells.Conclusion:TAX-NLC is more sensitive to KB cells,Ca2+concentration,the inhibition rate,multinucleated cells are closely associated.
