CAJ | 학술논문

目的：探索Beagle犬分离后的血清在不同保存条件和保存时间对血液生化指标检测的影响，保证长期毒性试验中检测结果的可靠性。方法选取Beagle犬6只，雌雄各半，采集Beagle犬静脉血，制备血清后，分别在1 h内、冰箱（2～8）℃保存2、5、8、12 h、冰盒（2～10）℃中进行运输保存2、5、8 h、冰箱－20℃保存1、3、5 d对丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、总蛋白（TP）、白蛋白（ALB）、尿素（UREA）、肌酐（CREA）、葡萄糖（GLU）、总胆固醇（TCHO）、总胆红素（TBIL）肌酸激酶（CK）、γ－谷氨酰基转移酶（GGT）、钙（CA）、乳酸脱氢酶（LDH）、磷（P）、高密度脂蛋白胆固醇（HDL－C）、低密度脂蛋白胆固醇（LDL－C）、甘油三酯（TG）、钠（Na＋）／钾（K＋）／氯（Cl －）进行检测。结果（2～8）℃冰箱保存12 h内和（2～10）℃冰盒保存8 h内的血液生化值均无差异；与血清分离后1 h内结果比较，－20℃保存5 d的LDL－C差异有显著性（ P＜0．05）且变动百分率为39％， CK、LDH值有逐渐增大的趋势，但无统计学意义（P＞0．05），LDH的变动百分率达41％。结论根据Beagle犬血液标本在不同保存条件和保存时间对血液生化指标检测结果的影响的试验结果，在制备血清后，应该在1h内进行检测，如未能在样本制备后1 h内检测，应将样本置于（2～8）℃环境并在12 h内进行检测，或置于约－20℃环境并在3 d内进行检测。要对血清标本进行运输的，应放置于冰盒（2～10℃）中，并在8 h内进行检测。
목적：탐색Beagle견분리후적혈청재불동보존조건화보존시간대혈액생화지표검측적영향，보증장기독성시험중검측결과적가고성。방법선취Beagle견6지，자웅각반，채집Beagle견정맥혈，제비혈청후，분별재1 h내、빙상（2～8）℃보존2、5、8、12 h、빙합（2～10）℃중진행운수보존2、5、8 h、빙상－20℃보존1、3、5 d대병안산안기전이매（ALT）、천문동안산안기전이매（AST）、감성린산매（ALP）、총단백（TP）、백단백（ALB）、뇨소（UREA）、기항（CREA）、포도당（GLU）、총담고순（TCHO）、총담홍소（TBIL）기산격매（CK）、γ－곡안선기전이매（GGT）、개（CA）、유산탈경매（LDH）、린（P）、고밀도지단백담고순（HDL－C）、저밀도지단백담고순（LDL－C）、감유삼지（TG）、납（Na＋）／갑（K＋）／록（Cl －）진행검측。결과（2～8）℃빙상보존12 h내화（2～10）℃빙합보존8 h내적혈액생화치균무차이；여혈청분리후1 h내결과비교，－20℃보존5 d적LDL－C차이유현저성（ P＜0．05）차변동백분솔위39％， CK、LDH치유축점증대적추세，단무통계학의의（P＞0．05），LDH적변동백분솔체41％。결론근거Beagle견혈액표본재불동보존조건화보존시간대혈액생화지표검측결과적영향적시험결과，재제비혈청후，응해재1h내진행검측，여미능재양본제비후1 h내검측，응장양본치우（2～8）℃배경병재12 h내진행검측，혹치우약－20℃배경병재3 d내진행검측。요대혈청표본진행운수적，응방치우빙합（2～10℃）중，병재8 h내진행검측。
Objective Beagle dogs are commonly used animal for drug safety evaluation .As the necessary parameters , blood biochemical indicators are detected in acute or chronic toxicity tests .This study aims at assessing the influence of different preservation conditions and different preservation time on blood biochemical indicators to ensure the reliability of test results of long-term toxicity assessment .Methods Six Beagle dogs (3 males and 3 females) were used in this study .After collection and preparation of serum samples , biochemical indicators were detected after preservation in refrigerator at 2-8℃for 1, 2, 5, 8, and 12 hours;after preservation in ice transportation boxes at 2-10℃for 2, 5, and 8 hours;and after preservation in refrigerator at -20℃ for 1, 3, and 5 days.The biochemical indicators included alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) and alkaline phosphatase ( ALP ) , total protein ( TP ) , albumin (propagated), urea, creatinine (CREA), glucose (GLU), total cholesterol (TCHO), total bilirubin (TBIL), creatine kinase ( CK ) , gamma pancreatic acyl transferase ( GGT ) , calcium ( CA ) , lactate dehydrogenase ( LDH ) , phosphorus ( P) , high-density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol ( LDL-C) , triglyceride ( TG) , sodium ( Na+) , potassium ( K+) and chloride ( Cl -) .Results Compared with the results of samples preserved for 1 hour, the LDL-C result of that preserved in refrigerator at -20℃for 5 days was significantly increased (P<0.05), and the coefficient of variation was 39%.The CK and LDH results of specimens preserved in refrigerator at -20℃ for 5 days was increased but not significantly ( P>0.05 ) , and the coefficient of variation of LDH was 41%.Conclusions According to the test results of blood biochemical indicators in the Beagle dogs detected after different preservation conditions and different preservation time in this study , detection test should be done within 1 hour, if not, detection should be done within 12 hours for the samples preserved at 2~8℃, or within 3 days for the sample preserved at -20℃.For transportation of serum samples , the serum samples should be placed in the ice box at 2~10℃, and detection test should be done within 8 hours .