CAJ | 학술논문

动物双歧杆菌A6是一株具有较强耐受性能的双歧杆菌菌株。本研究利用动物实验评价该菌株的缓解便秘作用。将Balb/c雄性小鼠随机分为空白组、模型组、A6不同剂量组。模型组、A6组使用复方地芬诺酯建立小鼠便秘模型，之后三组模型鼠分别给予低、中、高剂量(1×106、1×108、1×1010CFU/mL)的A6活菌液0.1mL/(kg·d)，空白组和模型组灌胃0.1mL/(kg·d)(以体质量计)质量分数10%的脱脂乳。利用墨汁推进方法评价排便情况。动物双歧杆菌A6给药7d后，便秘小鼠首次排便时间缩短，排便粒数和质量明显增加，粪便水分含量提高；连续15d灌胃动物双歧杆菌A6，便秘小鼠小肠墨汁推进率也得到显著增加。结果表明，动物双歧杆菌A6具有良好的润肠通便功能。
동물쌍기간균A6시일주구유교강내수성능적쌍기간균균주。본연구이용동물실험평개해균주적완해편비작용。장Balb/c웅성소서수궤분위공백조、모형조、A6불동제량조。모형조、A6조사용복방지분낙지건립소서편비모형，지후삼조모형서분별급여저、중、고제량(1×106、1×108、1×1010CFU/mL)적A6활균액0.1mL/(kg·d)，공백조화모형조관위0.1mL/(kg·d)(이체질량계)질량분수10%적탈지유。이용묵즙추진방법평개배편정황。동물쌍기간균A6급약7d후，편비소서수차배편시간축단，배편립수화질량명현증가，분편수분함량제고；련속15d관위동물쌍기간균A6，편비소서소장묵즙추진솔야득도현저증가。결과표명，동물쌍기간균A6구유량호적윤장통편공능。
Biifdobacterium.animalis subsp.Lactis A6 is a probiotic strain which has strong probiotic activity. To evaluate the defecating function of this strain, male Balb/c mice were randomly divided into control group, model control group, positive control group and 3 dosages group. Control group as well as model control group were orally administered with 10% skim milk liquid 0.1mL/(kg·d) dose once a day. SelectB. animalis subsp. And mice of A6 dosage group were administered with different concentrations (1×106、1×108、1×1010 CFU/mL) at 0.1 mL/(kg·d). Mice constipation model was established by using compound diphenoxylate. The mice administrated with B. animalis subsp.LactisA6for7dayshadtheshortertimeofdefecationoftheifrstblackfeces,andthenumber and weight of mice feces within 5 hours were increased significantly while water content in the feces were also increased; when the mice were administered withB. animalis subsp.Lactis A6 stain for 15 days, the ink propelling rates were raised. The results verify thatB. animalis subsp. Lactis A6 strain has the potential favorable defecation function in constipation mice.