CAJ | 학술논문

活菌数检测是益生菌培养过程分析的重要环节。传统以平板稀释法检测益生菌活菌数费时费力，且无法实现实时监测。本文探索了电容型活细胞在线检测器在双歧杆菌BBMN68培养过程中的应用效果，评价其检测活细胞量的可行性。研究表明，在菌体发酵生长的对数期，活细胞在线检测器的电容值与长双歧杆菌BBMN68活细胞量之间存在良好的线性关系，其线性相关系数为0.992，比吸光值法更准确地反映发酵体系中活细胞量的情况，在稳定期与衰亡期也表现出了良好的相关性。比平板计数法更快速，可以实现实时监控。电导型活细胞检测器为益生菌培养过程在线检测提供了一种简便、精确的方法。
활균수검측시익생균배양과정분석적중요배절。전통이평판희석법검측익생균활균수비시비력，차무법실현실시감측。본문탐색료전용형활세포재선검측기재쌍기간균BBMN68배양과정중적응용효과，평개기검측활세포량적가행성。연구표명，재균체발효생장적대수기，활세포재선검측기적전용치여장쌍기간균BBMN68활세포량지간존재량호적선성관계，기선성상관계수위0.992，비흡광치법경준학지반영발효체계중활세포량적정황，재은정기여쇠망기야표현출료량호적상관성。비평판계수법경쾌속，가이실현실시감공。전도형활세포검측기위익생균배양과정재선검측제공료일충간편、정학적방법。
An on-line viable-cell mass sensor was used to monitor the Viable cells of Bifidobacterium longum BBMN68 fermentation process. In this paper, this new method that based on the capacitive principle was employed. As a result, the relationship between the number of colony forming units(CFU) and capacitance with a correlation coefifcient(R) of 0.992 was achieved and data showed a good reproducibility under the same culture conditions. Compared with the results determined by absorbency method , the capacitive method is more reliable. Therefore, it could be concluded that a capacitance probe was a simple as well as a accurate tool inBiifdobacterium longum BBMN68 cultivation process.