CAJ | 학술논문

1型糖尿病(T1DM)是一种慢性代谢疾病，主要表现为胰岛素分泌量较正常情况下降，会对人体的多个器官和系统造成持续性的损伤．关于糖尿病的横向研究发现糖尿病患者相比于正常人存在着显著的脑萎缩，但关于糖尿病引起的脑萎缩随时间发生进行性改变的研究比较少见．实验采用腹腔注射链脲佐菌素(STZ)来诱导建立大鼠的1型糖尿病模型，运用磁共振成像(MRI)的方法对萎缩的脑区进行定位并在造模后12周和20周两个时间点对脑萎缩的程度进行对比分析，然后运用组织化学染色的方法观察在MRI上出现进行性萎缩的脑区中的神经元所发生的病理改变．MRI的结果表明：STZ诱导的T1DM大鼠相比于正常对照组大鼠出现了显著性的全脑体积、灰质体积和白质体积的萎缩，并且在多个白质脑区和灰质脑区均出现了萎缩程度随着病程的延长而逐渐加重．组织化学染色的结果发现， STZ诱导的T1DM大鼠相对于正常对照组大鼠在体感皮层、运动皮层和海马CA3区，均出现明显的神经元萎缩现象．
1형당뇨병(T1DM)시일충만성대사질병，주요표현위이도소분비량교정상정황하강，회대인체적다개기관화계통조성지속성적손상．관우당뇨병적횡향연구발현당뇨병환자상비우정상인존재착현저적뇌위축，단관우당뇨병인기적뇌위축수시간발생진행성개변적연구비교소견．실험채용복강주사련뇨좌균소(STZ)래유도건립대서적1형당뇨병모형，운용자공진성상(MRI)적방법대위축적뇌구진행정위병재조모후12주화20주량개시간점대뇌위축적정도진행대비분석，연후운용조직화학염색적방법관찰재MRI상출현진행성위축적뇌구중적신경원소발생적병리개변．MRI적결과표명：STZ유도적T1DM대서상비우정상대조조대서출현료현저성적전뇌체적、회질체적화백질체적적위축，병차재다개백질뇌구화회질뇌구균출현료위축정도수착병정적연장이축점가중．조직화학염색적결과발현， STZ유도적T1DM대서상대우정상대조조대서재체감피층、운동피층화해마CA3구，균출현명현적신경원위축현상．
Type 1 diabetes mellitus (T1DM) is a chronic metabolic disease characterized by insulin deficiency. Chronic T1DM causes damages to multiple organs. Numerous cross-sectional studies have shown that T1DM patients had significant cerebral atrophy, compared to normal subjects. However, few previous studies investigated progressive changes of cerebral atrophy over time in T1DM. In this study, a rat model of T1DM was established by a single dose of intraperitoneal injection of streptozotocin (STZ). Magnetic resonance imaging (MRI) was employed to measure the volumetric changes of the brain at 12 weeks and 20 weeks after STZ induction to follow the progressive brain atrophy assessment between these two time points. Histochemical staining was used assess neuropathologic changes in the brain regions showing progressive atrophy. The MRI results demonstrated that the STZ-treated rats had significantly reduced volume of grey matter (GM), white matter (WM) and whole brain, as compared to control. Voxel-wise analysis revealed significant effect of group×time interaction in multiple GM and WM regions. Results of Nissl staining and hematein-eosin staining (HE) indicate significant neuronal abnormality in the brain regions showing progressive atrophy, including somatosensory cortex, motor cortex and hippocampal CA3 region.