CAJ | 학술논문

目的：探讨椎间失稳环境下软骨终板蛋白多糖的变化。方法选取48只6月龄日本大耳白兔,体重(2.50±0.15)kg,均在相同环境条件下喂养,随机分为对照组和实验组,每组各24只；两组兔均进行椎间手术失稳。实验组在手术失稳2个月后用椎弓根螺丝钉和张力钢丝弹性内固定的方法进行L6/7的稳定性重建,对照组不进行稳定性重建。两组分别在内固定术后2、4、6个月取材对椎间盘软骨终板蛋白多糖进行检测,比较不同时间软骨终板蛋白多糖水平变化。结果①组内比较,两组术后2、4、6个月蛋白多糖灰度及光密度值比较,差异均有统计学意义(P<0.05)。对照组术后4个月蛋白多糖灰度低于同组术后2个月,光密度值高于术后2个月,差异均有高度统计学意义(P<0.01)；对照组术后6个月蛋白多糖灰度低于同组术后4个月,光密度值高于术后4个月,差异均有高度统计学意义(P<0.01)。实验组术后4个月蛋白多糖灰度高于同组术后2个月,光密度值低于术后2个月,差异均有高度统计学意义(P<0.01)；实验组术后6个月蛋白多糖灰度高于同组术后4个月,光密度值低于术后4个月,差异均有高度统计学意义(P<0.01)。②组间比较,实验组术后6个月蛋白多糖灰度高于对照组,光密度值低于对照组,差异均有统计学意义(P<0.05)。结论弹性内固定能有效地阻止椎间盘软骨终板蛋白多糖的退变,而且有部分恢复的征象,它为进一步进行其他治疗创造了必要条件。
목적：탐토추간실은배경하연골종판단백다당적변화。방법선취48지6월령일본대이백토,체중(2.50±0.15)kg,균재상동배경조건하위양,수궤분위대조조화실험조,매조각24지；량조토균진행추간수술실은。실험조재수술실은2개월후용추궁근라사정화장력강사탄성내고정적방법진행L6/7적은정성중건,대조조불진행은정성중건。량조분별재내고정술후2、4、6개월취재대추간반연골종판단백다당진행검측,비교불동시간연골종판단백다당수평변화。결과①조내비교,량조술후2、4、6개월단백다당회도급광밀도치비교,차이균유통계학의의(P<0.05)。대조조술후4개월단백다당회도저우동조술후2개월,광밀도치고우술후2개월,차이균유고도통계학의의(P<0.01)；대조조술후6개월단백다당회도저우동조술후4개월,광밀도치고우술후4개월,차이균유고도통계학의의(P<0.01)。실험조술후4개월단백다당회도고우동조술후2개월,광밀도치저우술후2개월,차이균유고도통계학의의(P<0.01)；실험조술후6개월단백다당회도고우동조술후4개월,광밀도치저우술후4개월,차이균유고도통계학의의(P<0.01)。②조간비교,실험조술후6개월단백다당회도고우대조조,광밀도치저우대조조,차이균유통계학의의(P<0.05)。결론탄성내고정능유효지조지추간반연골종판단백다당적퇴변,이차유부분회복적정상,타위진일보진행기타치료창조료필요조건。
Objective To investigate the change of cartilage endplate protein polysaccharide in the condition of inter-vertebral instability. Methods 48 white macrotia rabbits of 6 months old with (2.50±0.15) kg weight were fed in the same environment and randomly divided into the control group and the experiment group, with 24 rabbits in each group. Rabbits in the two groups were all given intervertebral instability surgery. Rabbits in experiment group were re-constructed the L6/7 stability with pedicle screw and tension wire elastic internal fixation 2 months after the interverte-bral instability surgery. Rabbits in control group were not reconstructed the L6/7 stability. The intervertebral disc carti-lage endplate protein polysaccharide were detected in the two groups 2, 4, 6 months after the internal fixationi;the car-tilage endplate proteoglycan level among different time were compared. Results ①The differences of protein polysac-charide grayscale and optical density value in the two groups 2, 4, 6 months after the fixation operation by variance analysis were statistically significant (P<0.05). The protein polysaccharide grayscale 4 months after fixation operation in control group were lower than those 2 months after fixation operation in the same term, the optical density values 4 the same term, the optical density values 6 months after fixation operation in control group were higher than those 4 months after fixation operation in the same term, the differences were statistically significant (P< 0.01). The protein polysaccharide grayscale 4 months after fixation operation in experiment group were higher than those 2 months after fixation operation in the same term, the optical density values 4 months after fixation operation in experiment group were lower than those 2 months after fixation operation in the same term, the differences were statistically significant (P<0.01);the protein polysaccharide grayscale 6 months after fixation operation in experiment group were higher than those 4 months after fixation operation in the same term, the optical density values 6 months after fixation operation in exper-iment group were lower than those 4 months after fixation operation in the same term, the differences were statistically significant (P< 0.01). ②The protein polysaccharide grayscale 6 months after fixation operation in experiment group were higher than those in control group, the optical density values 6 months after fixation operation in experiment group was lower than those in the control group, the differences were statistically significant (P<0.05). Conclusion The dy-namic internal fixation can effectively prevent the degeneration of the proteoglycan cartilage endplate and partly recover from the degeneration. It can create the necessary condition for the other treatment.