微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2015年
3期
5-8
,共4页
石玉婷%周陈陈%王镁%于世家
石玉婷%週陳陳%王鎂%于世傢
석옥정%주진진%왕미%우세가
糖肾安煎剂%胰激肽原酶肠溶片%激光散斑成像技术%微循环血流量%糖尿病大鼠
糖腎安煎劑%胰激肽原酶腸溶片%激光散斑成像技術%微循環血流量%糖尿病大鼠
당신안전제%이격태원매장용편%격광산반성상기술%미순배혈류량%당뇨병대서
Tang shenan decoction%Pancreatic kininogenase enteric-coated tablets%Laser speckle imaging technology%Microcirculatory blood flow%Diabetic rat
目的：：应用激光散斑成像(LSI)技术观察中药复方糖肾安煎剂对早期糖尿病(DM)大鼠耳廓及肾脏血流量的影响。方法：30只 SD 大鼠,采用高糖高脂饮食喂饲4周并一次性腹腔注射小剂量链脲佐菌素(STZ,35mg/kg)建立 DM 大鼠模型,将成模的28只大鼠随机分为 DM 模型组(n=9)、西药治疗组(胰激肽原酶肠溶片治疗,n=9)和中药治疗组(糖肾安煎剂治疗,n=10),另选10只 SD 大鼠作为正常对照组。药物治疗8周后,用 moor FLPI-2全景激光灌注成像仪监测各组大鼠耳廓及肾脏微循环血流量变化。结果：DM 模型组大鼠耳廓及肾脏血流量为133.42±49.58LSPU 和297.19±39.43LSPU,正常对照组为70.04±7.28LSPU 和239.27±33.27LSPU；中药治疗组为77.11±14.16LSPU 和253.6±35.57LSPU,西药治疗组为82.71±14.03LSPU 和248.96±39.26LSPU。DM 模型组上述检测值均高于正常对照组(P <0.01)；中药治疗组和西药治疗组上述检测值均显著低于 DM 模型组(P <0.01)；中药治疗组、西药治疗组和正常对照组组间血流量差异无统计学意义(P >0.05)。结论：糖肾安煎剂能明显减轻早期 DM 大鼠微循环障碍引起的微血管血流量增加,其效果与胰激肽原酶相当。
目的：：應用激光散斑成像(LSI)技術觀察中藥複方糖腎安煎劑對早期糖尿病(DM)大鼠耳廓及腎髒血流量的影響。方法：30隻 SD 大鼠,採用高糖高脂飲食餵飼4週併一次性腹腔註射小劑量鏈脲佐菌素(STZ,35mg/kg)建立 DM 大鼠模型,將成模的28隻大鼠隨機分為 DM 模型組(n=9)、西藥治療組(胰激肽原酶腸溶片治療,n=9)和中藥治療組(糖腎安煎劑治療,n=10),另選10隻 SD 大鼠作為正常對照組。藥物治療8週後,用 moor FLPI-2全景激光灌註成像儀鑑測各組大鼠耳廓及腎髒微循環血流量變化。結果：DM 模型組大鼠耳廓及腎髒血流量為133.42±49.58LSPU 和297.19±39.43LSPU,正常對照組為70.04±7.28LSPU 和239.27±33.27LSPU；中藥治療組為77.11±14.16LSPU 和253.6±35.57LSPU,西藥治療組為82.71±14.03LSPU 和248.96±39.26LSPU。DM 模型組上述檢測值均高于正常對照組(P <0.01)；中藥治療組和西藥治療組上述檢測值均顯著低于 DM 模型組(P <0.01)；中藥治療組、西藥治療組和正常對照組組間血流量差異無統計學意義(P >0.05)。結論：糖腎安煎劑能明顯減輕早期 DM 大鼠微循環障礙引起的微血管血流量增加,其效果與胰激肽原酶相噹。
목적：：응용격광산반성상(LSI)기술관찰중약복방당신안전제대조기당뇨병(DM)대서이곽급신장혈류량적영향。방법：30지 SD 대서,채용고당고지음식위사4주병일차성복강주사소제량련뇨좌균소(STZ,35mg/kg)건립 DM 대서모형,장성모적28지대서수궤분위 DM 모형조(n=9)、서약치료조(이격태원매장용편치료,n=9)화중약치료조(당신안전제치료,n=10),령선10지 SD 대서작위정상대조조。약물치료8주후,용 moor FLPI-2전경격광관주성상의감측각조대서이곽급신장미순배혈류량변화。결과：DM 모형조대서이곽급신장혈류량위133.42±49.58LSPU 화297.19±39.43LSPU,정상대조조위70.04±7.28LSPU 화239.27±33.27LSPU；중약치료조위77.11±14.16LSPU 화253.6±35.57LSPU,서약치료조위82.71±14.03LSPU 화248.96±39.26LSPU。DM 모형조상술검측치균고우정상대조조(P <0.01)；중약치료조화서약치료조상술검측치균현저저우 DM 모형조(P <0.01)；중약치료조、서약치료조화정상대조조조간혈류량차이무통계학의의(P >0.05)。결론：당신안전제능명현감경조기 DM 대서미순배장애인기적미혈관혈류량증가,기효과여이격태원매상당。
Objective:To observe the effect of tang shenan decoction on microcirculation blood flow in early diabetic rat auricle and kidney by using laser speckle imaging (LSI)technology.Method:30 SD rats were choosen to establish the diabetic rats models by high-lipids and high-sucrose diets for 4 th week and then intraperitoneal injection of one-time (STZ,35mg/kg),and then they were randomly divided into DM model group(n=9),western medicine-treated group (n = 9 )and chinese medicine-treated group(n = 10),another 10 SD rats were served as the control group.All groups were given relevant medicinal intragastrically.The changes of rats auricle and kidney microcircula-tion blood flow were detected by a Moor-full field laster perfusion imager (Moor-FLPI)at the end of 8 th week.Re-sults:The rats aurical and kidney blood flow of DM model were 133.42±49.58LSPU and 297.1 9.85±39.43LSPU. The control group were 70.04±7.28LSPU and 239.27±33.27LSPU.Both chinese medicine-treated group 77.1 1± 14.1 6LSPU,253.6 ±35.57 LSPU and western medicine-treated group 82.71 ±14.03LSPU,248.96 ±39.26 LSPU were obviously lower than the DM model group,and the differences were significant(P <0.01),but we found no statistical difference among chinese medicine-treated group、western medicine-treated group and the control group (P >0.05).Conclusion:Tang shenan decoction have the equivalent efficacy with pancreatic kininogenase in reducing the blood flow of rats aurical and kidney which was caused by early-stage diabetic.
