CAJ | 학술논문

目的：探讨人β-防御素-2（HBD-2）在肝细胞癌（HCC）中的表达及其意义。方法：收集2011年8月-2014年10月手术切除的原发性肝癌标本45例，肝癌癌旁组织30例，正常肝组织20例，采用免疫组化法检测各标本中HBD-2蛋白表达情况，采用聚合酶链反应（PCR）、实时聚合酶链反应（Real-Time PCR）检测HBD-2 mRNA表达情况，同时采用原位末端标记法（TUNEL法）测定癌组织的细胞凋亡指数。结果：HBD-2 mRNA及HBD-2蛋白在肝癌组织、癌旁组织和正常肝组织中的表达情况比较差异均有统计学意义（P<0.05）。HBD-2蛋白表达阳性的肝癌组织的细胞凋亡指数高于HBD-2蛋白表达阴性的肝癌组织，比较差异有统计学意义（P<0.01）。结论：HBD-2在肝细胞癌组织中低表达，HBD-2可能通过影响肝癌细胞的凋亡而起作用。
목적：탐토인β-방어소-2（HBD-2）재간세포암（HCC）중적표체급기의의。방법：수집2011년8월-2014년10월수술절제적원발성간암표본45례，간암암방조직30례，정상간조직20례，채용면역조화법검측각표본중HBD-2단백표체정황，채용취합매련반응（PCR）、실시취합매련반응（Real-Time PCR）검측HBD-2 mRNA표체정황，동시채용원위말단표기법（TUNEL법）측정암조직적세포조망지수。결과：HBD-2 mRNA급HBD-2단백재간암조직、암방조직화정상간조직중적표체정황비교차이균유통계학의의（P<0.05）。HBD-2단백표체양성적간암조직적세포조망지수고우HBD-2단백표체음성적간암조직，비교차이유통계학의의（P<0.01）。결론：HBD-2재간세포암조직중저표체，HBD-2가능통과영향간암세포적조망이기작용。
Objective:To investigate the expression and significance of HBD-2 in hepatocellular carcinoma(HCC). Method:45 specimens of HCC tissues,30 specimens of para-carcinoma tissues and 20 specimens of normal liver tissues in our hospital from August 2011 to October 2014 were collected.The expression situation of HBD-2 protein in the tissues was evaluated by immunohistochemistry,the expression situation of HBD-2 mRNA in the tissues was evaluated by PCR and Real-Time PCR, the cell apoptosis indexes in the tissues were detected by TUNEL methods.Result:The differences in the mean expression of HBD-2 protein and HBD-2 mRNA of hepatocellular carcinoma tissues,para-carcinoma tissues and normal liver tissues were statistically significant(P<0.05).The cell apoptosis indexes in the HCC tissues with positive expression of HBD-2 protein was higher than that in the HCC tissues with negative expression of HBD-2 protein,the difference was statistically significant(P<0.01). Conclusion:The expression of HBD-2 in HCC is low,it may play a part through the cell apoptosis of HCC.