CAJ | 학술논문

目的：探讨趋化因子 CCL27、CCL28及其受体 CCR10在小鼠癫痫急性期外周血中的表达变化。方法取正常及癫痫小鼠急性期不同时间点(10 min、30 min、1 h、2 h)外周血利用实时荧光定量 PCR(Real-time PCR)法检测毛果云香碱致癫痫发作小鼠急性期外周血中 CCL27、CCL28 mRNA 水平；同期取正常及癫痫急性期不同时间点(10 min、30 min、1 h、2 h)肝素抗凝外周血，提取淋巴细胞层后利用 FACSsort 型流式细胞仪检测外周血淋巴细胞 CCR10蛋白水平的表达变化。结果癫痫小鼠发作急性期外周血中 CCL27、CCL28 mRNA 水平、淋巴细胞中 CCR10蛋白表达水平均在癫痫发作2 h 高于对照组(P <0.05)。结论癫痫发作早期已出现外周血免疫功能紊乱，该病理变化可能与癫痫发病过程中的炎性反应和神经细胞的凋亡相关。
목적：탐토추화인자 CCL27、CCL28급기수체 CCR10재소서전간급성기외주혈중적표체변화。방법취정상급전간소서급성기불동시간점(10 min、30 min、1 h、2 h)외주혈이용실시형광정량 PCR(Real-time PCR)법검측모과운향감치전간발작소서급성기외주혈중 CCL27、CCL28 mRNA 수평；동기취정상급전간급성기불동시간점(10 min、30 min、1 h、2 h)간소항응외주혈，제취림파세포층후이용 FACSsort 형류식세포의검측외주혈림파세포 CCR10단백수평적표체변화。결과전간소서발작급성기외주혈중 CCL27、CCL28 mRNA 수평、림파세포중 CCR10단백표체수평균재전간발작2 h 고우대조조(P <0.05)。결론전간발작조기이출현외주혈면역공능문란，해병리변화가능여전간발병과정중적염성반응화신경세포적조망상관。
Objective To investigate the expression of chemokine CCL27,CCL28 and their receptor CCR10 in mouse periph-eral blood in the acute phase of epilepsy.Methods The peripheral blood of acute epileptic mice at different time points(10 min,30 min,1 h,2 h)was collected,real-time PCR was used to detect the mRNA expression level of CCL27 and CCL28.The heparin anti-coagulation peripheral blood at the same time points(10 min,30 min,1 h,2 h)of normal and acute phase of epileptic mice were col-lected and flow cytometry was used to investigate the expression of CCR10 in peripheral blood lymphocyte.Results The mRNA expression level of CCL27,CCL28 in peripheral blood and the expression of CCR10 in lymphocytes were found significantly in-creased at 2 h in epileptic mice than those of normal(P <0.01).Conclusion The immune function disorder occured in peripheral blood in early epilepsic pathological process and might be associated with the subsequent inflammatory reaction and neuron apoptosis.