CAJ | 학술논문

目的：通过观测不同浓度转化生长因子β1（transforming growth factors β1，TGF‐β1）对种植体表面成骨细胞中骨钙素（osteocalcin ，OC）表达的影响，以探讨其对种植体表面成骨细胞分化的作用。方法专用钛片模拟种植体植入后的体内环境，体外培养成骨细胞接种于钛片表面，分为实验组和对照组，实验组分别加入0．5ng／ml、10ng／ml的 TGF‐β1，收集第1d、3d、7d培养后的细胞，对照组不加TGF‐β1。通过 RT‐PCR检测不同培养时间成骨细胞中 OCmRNA 的表达情况。所有数据采用SPSS 16．0软件作统计学处理，然后比较分析各组间差异程度，P ＜0．05认为差异有显著性意义。结果浓度为0．5ng／ml外源性 TGF‐β1加入到接种于钛片表面的成骨细胞中后，显著增加了成骨细胞中骨钙素的表达（ P ＜0．05），而浓度为10ng／ml的外源性TGF‐β1可降低成骨细胞中骨钙素的表达（ P ＜0．05）。结论外源性TGF‐β1对接种在模拟体内种植体钛片表面成骨细胞中OC表达的作用与其浓度相关。
목적：통과관측불동농도전화생장인자β1（transforming growth factors β1，TGF‐β1）대충식체표면성골세포중골개소（osteocalcin ，OC）표체적영향，이탐토기대충식체표면성골세포분화적작용。방법전용태편모의충식체식입후적체내배경，체외배양성골세포접충우태편표면，분위실험조화대조조，실험조분별가입0．5ng／ml、10ng／ml적 TGF‐β1，수집제1d、3d、7d배양후적세포，대조조불가TGF‐β1。통과 RT‐PCR검측불동배양시간성골세포중 OCmRNA 적표체정황。소유수거채용SPSS 16．0연건작통계학처리，연후비교분석각조간차이정도，P ＜0．05인위차이유현저성의의。결과농도위0．5ng／ml외원성 TGF‐β1가입도접충우태편표면적성골세포중후，현저증가료성골세포중골개소적표체（ P ＜0．05），이농도위10ng／ml적외원성TGF‐β1가강저성골세포중골개소적표체（ P ＜0．05）。결론외원성TGF‐β1대접충재모의체내충식체태편표면성골세포중OC표체적작용여기농도상관。
Objective By observing different concentration of transforming grow th factor beta 1 (trans‐forming growth factors beta 1 ,TGF‐β1) of the implant surface osteoblast in osteocalcin (osteocalcin ,OC) expression and the effect of in order to investigate its effect on osteoblast differentiation on the surface of the implant .Methods Osteoblast were divided into two groups :the control group and the induced group . The cells were collected on Days 1 ,3 ,7after treatment .The expression of osteocalcin(BSP) mRNA were detected by reverse transcription‐polymerase chain reaction (RT‐PCR ) .All the data were analyzed by SPSS16 .0 software .Results The extraneous (0 .5ng/ml) can increase the gene expressions of osteocalcin in osteoblasts on implant surfaces( P <0 .05) ,but reduced when treatment with 10ng/ml of TGF‐β1( P <0 . 05) .Conclusion Exogenous TGF‐β1 docking in simulated body titanium implant surface OC expression in osteoblast function is related to its concentration .