重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
24期
3341-3344
,共4页
多态性,单核苷酸%血脂异常%NPC1L1%人体质量指数
多態性,單覈苷痠%血脂異常%NPC1L1%人體質量指數
다태성,단핵감산%혈지이상%NPC1L1%인체질량지수
polymorphism,single nucleotide%dyslipidemias%niemann-pick c1-like 1%body mass index
目的：分析 NPC1L1基因1735 C>G 多态性分布特征及其与血脂、肥胖的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对409例汉族人群进行 NPC1L1基因多态性分型,比较不同基因型间血脂水平、BMI 的差异。结果CC,CG,GG 基因型的分布频率分别为39.40%、46.00%、14.60%；等位基因 C 和 G 频率分别为62.35%和37.65%。3种基因型组间的 LDL-C、ApoB 水平和 BMI 的差异有统计学意义(P <0.05),G 等位基因携带者(GC/GG 基因型)较 CC 型有更高的 LDL-C、ApoB 水平(P <0.05)。血脂谱和 BMI 与多种环境因素相关。结论NPC1L1基因1735 C>G 多态性与血脂水平和 BMI 相关,G 等位基因可能是高 LDL-C 水平和肥胖的一个危险因素。
目的：分析 NPC1L1基因1735 C>G 多態性分佈特徵及其與血脂、肥胖的關繫。方法採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法,對409例漢族人群進行 NPC1L1基因多態性分型,比較不同基因型間血脂水平、BMI 的差異。結果CC,CG,GG 基因型的分佈頻率分彆為39.40%、46.00%、14.60%；等位基因 C 和 G 頻率分彆為62.35%和37.65%。3種基因型組間的 LDL-C、ApoB 水平和 BMI 的差異有統計學意義(P <0.05),G 等位基因攜帶者(GC/GG 基因型)較 CC 型有更高的 LDL-C、ApoB 水平(P <0.05)。血脂譜和 BMI 與多種環境因素相關。結論NPC1L1基因1735 C>G 多態性與血脂水平和 BMI 相關,G 等位基因可能是高 LDL-C 水平和肥胖的一箇危險因素。
목적：분석 NPC1L1기인1735 C>G 다태성분포특정급기여혈지、비반적관계。방법채용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법,대409례한족인군진행 NPC1L1기인다태성분형,비교불동기인형간혈지수평、BMI 적차이。결과CC,CG,GG 기인형적분포빈솔분별위39.40%、46.00%、14.60%；등위기인 C 화 G 빈솔분별위62.35%화37.65%。3충기인형조간적 LDL-C、ApoB 수평화 BMI 적차이유통계학의의(P <0.05),G 등위기인휴대자(GC/GG 기인형)교 CC 형유경고적 LDL-C、ApoB 수평(P <0.05)。혈지보화 BMI 여다충배경인소상관。결론NPC1L1기인1735 C>G 다태성여혈지수평화 BMI 상관,G 등위기인가능시고 LDL-C 수평화비반적일개위험인소。
Objective To analyze the distributional characteristics of NPC1L1 1 735 C>G polymorphism and the relation-ship between serum lipid levels and obesity in Guangxi Han population.Methods Genotyping of the NPC1L1 1 735 C>G poly-morphism of 409 cases of Han was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis in all subjects.The relationship of different genotypes in blood lipid levels and obesity were evaluated.Re-sults The frequency of CC,CG and GG genotypes was 39.40%,46.00% and 14.60% in Han.The frequency of C and G alleles was 62.35% and 37.65% in Han.The levels of BMI,LDL-C,ApoB was different among the three genotypes (P <0.05).The G al-lele carriers had higher serum LDL-C and ApoB levels than the G allele noncarriers (P <0.05 ).Serum lipid parameters and BMI were also correlated with some environmental factors.Conclusion The 1 735 C>G polymorphism in NPC1L1 gene may be correla-ted with serum lipid profiles and obesity,the G allele might increase the risk of hyperlipemia and obesity.
