CAJ | 학술논문

目的：通过建立幼兔大段腓骨切除模型,探讨再生的腓骨与健侧腓骨在组织形态、成骨能力、生物应力等方面是否存在差异。方法3月龄新西兰小白兔20只,随机编号。取右侧腓骨(1.5 cm),X 线监测其再生情况(15 d、30 d、60 d)。术后60 d 取再生及健侧同位置腓骨(1.5 cm)。苏木素-伊红(HE)染色观察两者组织形态的差异、碱性磷酸酶(ALP)染色检测其成骨能力、3点弯曲试验测定两组骨生物力学。结果X 线监测：15 d 缺损区两端有新骨形成,取骨区有少许模糊影；30 d 新生骨长入取骨区；60 d 取骨区形成完整的新骨。生物应力检测：两侧腓骨差异无统计学意义(P >0.05)；HE 染色、ALP 染色：两侧腓骨在组织形态、成骨能力上未见明显差异。结论幼兔腓骨切除后保留骨膜可原位再生腓骨,且再生腓骨与健侧腓骨在组织形态、成骨能力、生物应力无差异。
목적：통과건립유토대단비골절제모형,탐토재생적비골여건측비골재조직형태、성골능력、생물응력등방면시부존재차이。방법3월령신서란소백토20지,수궤편호。취우측비골(1.5 cm),X 선감측기재생정황(15 d、30 d、60 d)。술후60 d 취재생급건측동위치비골(1.5 cm)。소목소-이홍(HE)염색관찰량자조직형태적차이、감성린산매(ALP)염색검측기성골능력、3점만곡시험측정량조골생물역학。결과X 선감측：15 d 결손구량단유신골형성,취골구유소허모호영；30 d 신생골장입취골구；60 d 취골구형성완정적신골。생물응력검측：량측비골차이무통계학의의(P >0.05)；HE 염색、ALP 염색：량측비골재조직형태、성골능력상미견명현차이。결론유토비골절제후보류골막가원위재생비골,차재생비골여건측비골재조직형태、성골능력、생물응력무차이。
Objective To investigate the difference between anagenetic fibula with normal fibula in mechanical parameters, osteogenesis and tissue morphology,through establishing the model of fibula defect in rabbit.Methods Twenty New Zealand white rabbits were randomly divided.Model group were cuted the right fibula bone with 1.5 cm length.X-ray was used to observe the anagenetic fibula(half month,one month,two months).Stumped the anagenetic and normal fibula(1.5 cm)after two months. Three-point bending test was used to test the mechanical properties;alkaline phosphatase staining was checked the bone-formation ability;HE staining to check the tissue morphology.Results After half month a few new bone were formed at the edges of resec-tion area,one month later new bone were growed in the donor site,and after two month new fibula were completely formed.The three point bending test of fibula showed:there was no statistically significant difference between anagenetic fibula and normal fibu-la(P >0.05);alkaline phosphatase staining and HE staining showed the anagenetic fibula bone forming ability,histology had no sig-nificant difference compared with normal fibula.Conclusion Fibula were regenerated successfully after removing which retaining the periosteum.Compared with the normal fibula,there were no remarkable differences in mechanical parameters,osteogenesis and tissue morphology.