CAJ | 학술논문

目的：观察黄芩苷对动脉粥样硬化模型大鼠的主动脉壁细胞内核因子(NF-κB)、纤溶抑制物(TAFI)及血管紧张素转换酶2(ACE2)表达水平影响,探讨黄芩苷防治动脉粥样硬化机制。方法选取健康雄性 Wistar 大鼠作为实验研究对象,分为4组,每组10只,为假手术组、模型组、黄芩苷高剂量组(黄芩苷150 mg·kg-1·d-1)、黄芩苷低剂量组(黄芩苷100 mg·kg-1· d-1)),发色底物法测定血浆 TAFI 的活性,免疫组织化学法测定主动脉壁细胞内核因子 NF-κB,ACE2表达水平。结果与假手术组相比,其余组大鼠 TAFI、ACE2活性升高,NF-κB 灰度值降低(P <0.05)；黄芩苷高、低剂量组 TAFI、ACE2明显低于模型组, NF-κB 高于模型组(P <0.05)。结论黄芩苷可能通过下调 TAFI 水平,NF-κB 表达,上调 ACE2表达,发挥其抗动脉粥样硬化作用。
목적：관찰황금감대동맥죽양경화모형대서적주동맥벽세포내핵인자(NF-κB)、섬용억제물(TAFI)급혈관긴장소전환매2(ACE2)표체수평영향,탐토황금감방치동맥죽양경화궤제。방법선취건강웅성 Wistar 대서작위실험연구대상,분위4조,매조10지,위가수술조、모형조、황금감고제량조(황금감150 mg·kg-1·d-1)、황금감저제량조(황금감100 mg·kg-1· d-1)),발색저물법측정혈장 TAFI 적활성,면역조직화학법측정주동맥벽세포내핵인자 NF-κB,ACE2표체수평。결과여가수술조상비,기여조대서 TAFI、ACE2활성승고,NF-κB 회도치강저(P <0.05)；황금감고、저제량조 TAFI、ACE2명현저우모형조, NF-κB 고우모형조(P <0.05)。결론황금감가능통과하조 TAFI 수평,NF-κB 표체,상조 ACE2표체,발휘기항동맥죽양경화작용。
Objective To explore the effects of baicalin on the expression of TAFI,NF-κB,and ACE2 in the atherosclerotic rats and discuss the anti-atherosclerosis mechanisms of baicalin.Methods The subjects were healthy male Wistar rats.The rats were divided into sham operation group,model group,baicalin high dose group (1 50 mg·kg-1 ·d-1 )and baicalin low dose group (100 mg·kg-1 ·d-1 ).Plasma TAFI activity were detected with enzymatic assays.The expression of NF-κB and ACE2 were detec-ted with immunohistochemical staining.Results Compared with sham group,the rest groups of TAFI and ACE2 activity were sig-nificantly higher (P <0.05),and the expression of NF-κB,had significant decreased (P <0.05).Compared with the model group, the level of TAFI and ACE2 in baicalin drug group was significantly lower than the model group (P <0.05),and the expression of NF-κB,had significant increased.Conclusion Baicalin can reduce TAFI and ACE2 level,and upregulate the expression of NF-κB to play its role in anti atherosclerosis.