CAJ | 학술논문

本试验研究了超声辅助提取佛手瓜中总黄酮的工艺。在单因素试验基础上，考察乙醇浓度、超声温度、液料比和超声时间对佛手瓜总黄酮提取率的影响。根据Box-Benhnken中心组合试验设计原理，采用响应面法对佛手瓜总黄酮提取的关键参数进行了优化，建立了佛手瓜总黄酮提取率的数学模型。结果表明：4个因素对佛手瓜总黄酮提取率影响的大小顺序是超声温度＞超声时间＞液料比＞乙醇浓度。佛手瓜总黄酮的最佳提取工艺条件为：乙醇浓度69%、超声温度71℃、液料比25 mL/g、超声时间31 min，在此条件下总黄酮提取率为3.402%，与理论预测值相对误差为0.526%。与传统热水浸提法相比，提取率提高了12.69%。
본시험연구료초성보조제취불수과중총황동적공예。재단인소시험기출상，고찰을순농도、초성온도、액료비화초성시간대불수과총황동제취솔적영향。근거Box-Benhnken중심조합시험설계원리，채용향응면법대불수과총황동제취적관건삼수진행료우화，건립료불수과총황동제취솔적수학모형。결과표명：4개인소대불수과총황동제취솔영향적대소순서시초성온도＞초성시간＞액료비＞을순농도。불수과총황동적최가제취공예조건위：을순농도69%、초성온도71℃、액료비25 mL/g、초성시간31 min，재차조건하총황동제취솔위3.402%，여이론예측치상대오차위0.526%。여전통열수침제법상비，제취솔제고료12.69%。
The ultrasonic-assisted extraction conditions of total flavonoids from Sechium edule(Jacq.)Swartz were stud-ied.Single factors including alcohol concentration,ultrasonic temperature,the ratio of liquid to material and ultrasonic time were investigated.Response surface methodology with 4 factors and 3 levels followed by verification test was adopted and a second order quadratic equation was established.The results showed that the effect order of four factors on the total flavonoids yield was as follows:ultrasonic temperature,ultrasonic time,the ratio of liquid to material and alcohol concentration.The op-timum condition were alcohol concentration 69%,ultrasonic temperature 71℃,the ratio of liquid to material 25 mL/g and ul-trasonic time 31 min.Under the optimized conditions,the total flavonoids yield reached to 3.402 %,the relative error was 0.526% compared to the predictive value,which indicated the feasible model fitted well with the experimental data.Total flavonoids yield using ultrasonic-assisted extraction was 12.69%higher than that of traditional heat refluxing extraction.