重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
24期
3328-3330
,共3页
范祯祯%刘志艳%蔡宏斌%张翠杰%张旭东%张兰芳%梁成%葛朝明
範禎禎%劉誌豔%蔡宏斌%張翠傑%張旭東%張蘭芳%樑成%葛朝明
범정정%류지염%채굉빈%장취걸%장욱동%장란방%량성%갈조명
高压氧%颈内动脉%基底动脉%细胞凋亡%Caspase-3%Bcl-2%实时荧光定量 PCR
高壓氧%頸內動脈%基底動脈%細胞凋亡%Caspase-3%Bcl-2%實時熒光定量 PCR
고압양%경내동맥%기저동맥%세포조망%Caspase-3%Bcl-2%실시형광정량 PCR
hyperbaric Oxygen%internal carotid artery%basal artery%cell apoptosis%Caspase-3%Bcl-2%Real-time F.Q.PCR
目的：探讨高压氧(HBO)对兔颈内动脉和基底动脉内皮细胞 Caspase-3和 Bcl-2 mRNA 表达的影响。方法健康成年新西兰白兔24只,随机分为 HBO 组和对照组各12只。HBO 组每日持续暴露于2.2 ATA HBO 下60 min,连续3 d；对照组正常饲养,不予任何处理。实时荧光定量 PCR 法检测 Caspase-3、Bcl-2 mRNA 在2组颈内动脉和基底动脉内皮细胞的表达水平。结果HBO 可使颈内动脉内皮细胞 Caspase-3 mRNA 表达明显下降[(0.038±0.006)vs .(1.000±0.225)],Bcl-2 mRNA 表达明显增高[(1.877±0.169)vs .(1.000±0.364)]；使基底动脉内皮细胞 Caspase-3 mRNA 表达明显降低[(0.419±0.091)vs .(1.000±0.175)],Bcl-2 mRNA 的表达明显升高[(1.269±0.270)vs .(1.000±0.117)],差异均有统计学意义(P <0.01)。结论HBO 对兔脑血管内皮细胞凋亡的发生有抑制作用,其机制可能与 HBO 对促凋亡基因 Caspase-3的抑制和抗凋亡基因 Bcl-2的促进作用有关。
目的：探討高壓氧(HBO)對兔頸內動脈和基底動脈內皮細胞 Caspase-3和 Bcl-2 mRNA 錶達的影響。方法健康成年新西蘭白兔24隻,隨機分為 HBO 組和對照組各12隻。HBO 組每日持續暴露于2.2 ATA HBO 下60 min,連續3 d；對照組正常飼養,不予任何處理。實時熒光定量 PCR 法檢測 Caspase-3、Bcl-2 mRNA 在2組頸內動脈和基底動脈內皮細胞的錶達水平。結果HBO 可使頸內動脈內皮細胞 Caspase-3 mRNA 錶達明顯下降[(0.038±0.006)vs .(1.000±0.225)],Bcl-2 mRNA 錶達明顯增高[(1.877±0.169)vs .(1.000±0.364)]；使基底動脈內皮細胞 Caspase-3 mRNA 錶達明顯降低[(0.419±0.091)vs .(1.000±0.175)],Bcl-2 mRNA 的錶達明顯升高[(1.269±0.270)vs .(1.000±0.117)],差異均有統計學意義(P <0.01)。結論HBO 對兔腦血管內皮細胞凋亡的髮生有抑製作用,其機製可能與 HBO 對促凋亡基因 Caspase-3的抑製和抗凋亡基因 Bcl-2的促進作用有關。
목적：탐토고압양(HBO)대토경내동맥화기저동맥내피세포 Caspase-3화 Bcl-2 mRNA 표체적영향。방법건강성년신서란백토24지,수궤분위 HBO 조화대조조각12지。HBO 조매일지속폭로우2.2 ATA HBO 하60 min,련속3 d；대조조정상사양,불여임하처리。실시형광정량 PCR 법검측 Caspase-3、Bcl-2 mRNA 재2조경내동맥화기저동맥내피세포적표체수평。결과HBO 가사경내동맥내피세포 Caspase-3 mRNA 표체명현하강[(0.038±0.006)vs .(1.000±0.225)],Bcl-2 mRNA 표체명현증고[(1.877±0.169)vs .(1.000±0.364)]；사기저동맥내피세포 Caspase-3 mRNA 표체명현강저[(0.419±0.091)vs .(1.000±0.175)],Bcl-2 mRNA 적표체명현승고[(1.269±0.270)vs .(1.000±0.117)],차이균유통계학의의(P <0.01)。결론HBO 대토뇌혈관내피세포조망적발생유억제작용,기궤제가능여 HBO 대촉조망기인 Caspase-3적억제화항조망기인 Bcl-2적촉진작용유관。
Objective To investigate the effects of hyperbaric Oxygen (HBO)exposure on Caspase-3 and Bcl-2 mRNA ex-pressions in both internal carotid artery (ICA)and basal artery (BA)in rabbits.Methods Twenty-four healthy adult New Zealand rabbits were randomly divided into 2 groups:HBO group and the control group,with each group consisted of 12 animals.The rab-bits in the HBO group were exposed to HBO at 2.2 ATA for 60 minutes each day for 3 successive days.The rabbits in the control group were normally fed without any treatment.Real-time PCR was used to detect Caspase-3 and Bcl-2 mRNA expressions in both ICA and BA in 2 groups.Results HBO significantly decreased the Caspase-3 mRNA expression [(0.038 ±0.006 )vs .(1.000 ± 0.225)]and increased the Bcl-2 mRNA expression [(1.877 ±0.1 69)vs .(1.000 ±0.364)].In ICA,HBO similarly decreased the Caspase-3 mRNA expression [(0.41 9±0.091)vs .(1.000 ±0.1 75)]and increased the Bcl-2 mRNA expression [(1.269 ±0.270) vs .(1.000±0.1 1 7)]in BA.All the differences mentioned above were of statistical significance (P <0.01).Conclusion HBO ex-erts an inhibition effect on apoptosis in cerebral vascular endothelial cells.The mechanism may be related to inhibiting the expres-sion of Caspase-3 mRNA and promoting the expression of Bcl-2 mRNA.
