实用药物与临床
實用藥物與臨床
실용약물여림상
PRACTICAL PHARMACY AND CLINICAL REMEDIES
2015年
8期
946-950
,共5页
哈蟆油%腺嘌呤%星点设计-效应面法%HPLC%提取工艺
哈蟆油%腺嘌呤%星點設計-效應麵法%HPLC%提取工藝
합마유%선표령%성점설계-효응면법%HPLC%제취공예
Oviductus ranae%Adenine%Central composite design-response surface methodology%HPLC%Extraction technology
目的：采用星点设计-效应面法优选哈蟆油中腺嘌呤的提取工艺。方法采用HPLC测定腺嘌呤含量,以Inertsil ODS-SP Extend C18(250 mm ×4．6 mm,5．0μm)为色谱柱,流动相甲醇-水,梯度洗脱,流速1．0 mL/min,检测波长260 nm,柱温25℃,进样量10μL。以腺嘌呤为考察指标,通过星点设计-效应面法考察甲醇浓度、提取时间、提取温度对哈蟆油腺嘌呤提取工艺的影响。结果腺嘌呤对照品进样量在17．73~110．33μg/mL呈良好线形关系,回归方程为Y=0．1045 X+0．1001,R2=0．9996,平均回收率为97．84%,RSD为1．76%(n=6)。最佳提取工艺为甲醇浓度73%,提取时间68 min,提取温度61℃,哈蟆油得率为465．30μg/g。结论优选的哈蟆油腺嘌呤提取工艺简便、稳定。
目的：採用星點設計-效應麵法優選哈蟆油中腺嘌呤的提取工藝。方法採用HPLC測定腺嘌呤含量,以Inertsil ODS-SP Extend C18(250 mm ×4．6 mm,5．0μm)為色譜柱,流動相甲醇-水,梯度洗脫,流速1．0 mL/min,檢測波長260 nm,柱溫25℃,進樣量10μL。以腺嘌呤為攷察指標,通過星點設計-效應麵法攷察甲醇濃度、提取時間、提取溫度對哈蟆油腺嘌呤提取工藝的影響。結果腺嘌呤對照品進樣量在17．73~110．33μg/mL呈良好線形關繫,迴歸方程為Y=0．1045 X+0．1001,R2=0．9996,平均迴收率為97．84%,RSD為1．76%(n=6)。最佳提取工藝為甲醇濃度73%,提取時間68 min,提取溫度61℃,哈蟆油得率為465．30μg/g。結論優選的哈蟆油腺嘌呤提取工藝簡便、穩定。
목적：채용성점설계-효응면법우선합마유중선표령적제취공예。방법채용HPLC측정선표령함량,이Inertsil ODS-SP Extend C18(250 mm ×4．6 mm,5．0μm)위색보주,류동상갑순-수,제도세탈,류속1．0 mL/min,검측파장260 nm,주온25℃,진양량10μL。이선표령위고찰지표,통과성점설계-효응면법고찰갑순농도、제취시간、제취온도대합마유선표령제취공예적영향。결과선표령대조품진양량재17．73~110．33μg/mL정량호선형관계,회귀방정위Y=0．1045 X+0．1001,R2=0．9996,평균회수솔위97．84%,RSD위1．76%(n=6)。최가제취공예위갑순농도73%,제취시간68 min,제취온도61℃,합마유득솔위465．30μg/g。결론우선적합마유선표령제취공예간편、은정。
Objective To optimize the process of extracting adenine from oviductus ranae by central compos-ite design/response surface methodology. Methods The analysis was achieved with an Inertsil ODS-SP Extend C18 column (250 mm × 4. 6 mm,5. 0 μm) by gradient elution of methanol and water at 25 ℃. The flow rate was 1. 0 mL/min,injection volume was 10 μL,and detection wave length was set at 260 nm. The effects of influence factors such as methanol concentration,extraction time and extraction temperature on extraction rate of adenine was investigated using box-behnken design and response surface method. Results The results indicate that the calibration curves of adenine have good linearity over the range from 17. 73 to 110. 33 μg/mL with a regression equation of Y=0. 104 5 X+0. 100 1,R2 =0. 999 6. The mean recovery was 97. 84% (RSD=1. 76%,n=6). Optimum processing conditions were: the concentration of methanol 73%,the extraction time was 68 min,the extraction temperature was 61 ℃ as the rate of ade-nine was 465. 30 μg/g. Conclusion Optimized processing technology is simple and stable.
