CAJ | 학술논문

目的：建立动物模型观察脊髓损伤后的病理生理改变，寻找可直接判断脊髓损伤程度的方法，优选出治疗脊髓损伤的最佳方案。方法暴露大鼠L2-3脊髓后，于脊髓内注射0．5%布比卡因，评价大鼠行为学改变及脊髓形态学改变。观察并记录大鼠后肢行为学改变及脊髓神经元和周围组织的病理改变，评估脊髓损伤程度。结果与生理盐水注射组(对照组)相比，布比卡因注射组(观察组)大鼠脊髓损伤，表现为术后大鼠BBB评分降低，与对照组比较差异有统计学意义，病理HE染色可见脊髓神经元出现空泡变性及液化坏死。结论应用腰麻针进行大鼠脊髓穿刺并注射布比卡因的大鼠动物模型，具有稳定可控的脊髓损伤效果。
목적：건립동물모형관찰척수손상후적병리생리개변，심조가직접판단척수손상정도적방법，우선출치료척수손상적최가방안。방법폭로대서L2-3척수후，우척수내주사0．5%포비잡인，평개대서행위학개변급척수형태학개변。관찰병기록대서후지행위학개변급척수신경원화주위조직적병리개변，평고척수손상정도。결과여생리염수주사조(대조조)상비，포비잡인주사조(관찰조)대서척수손상，표현위술후대서BBB평분강저，여대조조비교차이유통계학의의，병리HE염색가견척수신경원출현공포변성급액화배사。결론응용요마침진행대서척수천자병주사포비잡인적대서동물모형，구유은정가공적척수손상효과。
Objective To observe the pathophysiological changes after spinal cord injury by establishing the animal model,and to find the method that can directly determine the extent of spinal cord injury and optimize the best treatment for the injury. Methods After the exposure of the L2-3 spinal cord of each rat,0. 5% bupivacaine was injec-ted,and the changes in behavior and morphology were evaluated. The behavior changes of each rat′s hind limb and the histopathological changes of spinal cord neurons and the surrounding were observed and recorded to provide a evalua-tion of the degree of SCI. Results The spinal cord injury in rats of bupivacaine group was more serious compared with that of control group,the BBB score of postoperative rats in bupivacaine group was lower than that of control group, and the pathological HE staining of spinal cord neurons in bupivacaine group appeared empty degeneration and liquefac-tion necrosis. Conclusion The model of rats that received spinal needle puncture and injected with bupivacaine has a more stable and controllable effects on spinal cord injury.