临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
CHINESE CLINICAL ONCOLOGY
2015年
8期
694-698
,共5页
Stomatin样蛋白2%三基序蛋白25%肝细胞癌
Stomatin樣蛋白2%三基序蛋白25%肝細胞癌
Stomatin양단백2%삼기서단백25%간세포암
Stomatin like protein 2%Tripartite motif-containing 25%Hepatocellular carcinoma
目的：探讨肝细胞癌（ HCC）组织中Stomatin样蛋白2（ STOML2）和三基序蛋白25（ TRIM25）的表达水平及其与临床病理特征的关系。方法采用免疫组化EnVision二步法检测75例HCC组织和61例癌旁正常肝组织中STOML2和TRIM25的表达强度，根据细胞内染色强度和阳性细胞数分级，分析STOML2、TRIM25的表达与HCC临床病理特征的关系及两者的相关性；同时采用Kaplan?Meier法分析STOML2、TRIM25不同表达强度的总生存期（ OS）。结果 STOML2和TRIM25在HCC组织中的高表达率分别为68?0％（51／75）和64?0％（48／75），均高于癌旁组织的24?6％（15／61）和37?7％（23／61），差异有统计学意义（ P＜0?01）；STOML2表达与肿瘤大小、分化程度、BCLC分期、门静脉癌栓和肝外转移有关，而TRIM25蛋白表达与治疗前甲胎蛋白（AFP）、分化程度和腹水均有关（P＜0?05），且STOML2与TRIM25表达呈正相关（r＝0?379， P＝0?001）；TRIM25高表达者的中位OS为21?0个月，低于低表达者的36?5个月（ P＜0?05）；但STOML2不同表达水平患者的中位OS的差异无统计学意义（ P＞0?05）。结论 STOML2和TRIM25在HCC组织中呈高表达，两者联合检测可用于评估肝癌的恶性表现、分化情况及肝转移程度，且TRIM25水平可能有助于生存预后的评估和预测。
目的：探討肝細胞癌（ HCC）組織中Stomatin樣蛋白2（ STOML2）和三基序蛋白25（ TRIM25）的錶達水平及其與臨床病理特徵的關繫。方法採用免疫組化EnVision二步法檢測75例HCC組織和61例癌徬正常肝組織中STOML2和TRIM25的錶達彊度，根據細胞內染色彊度和暘性細胞數分級，分析STOML2、TRIM25的錶達與HCC臨床病理特徵的關繫及兩者的相關性；同時採用Kaplan?Meier法分析STOML2、TRIM25不同錶達彊度的總生存期（ OS）。結果 STOML2和TRIM25在HCC組織中的高錶達率分彆為68?0％（51／75）和64?0％（48／75），均高于癌徬組織的24?6％（15／61）和37?7％（23／61），差異有統計學意義（ P＜0?01）；STOML2錶達與腫瘤大小、分化程度、BCLC分期、門靜脈癌栓和肝外轉移有關，而TRIM25蛋白錶達與治療前甲胎蛋白（AFP）、分化程度和腹水均有關（P＜0?05），且STOML2與TRIM25錶達呈正相關（r＝0?379， P＝0?001）；TRIM25高錶達者的中位OS為21?0箇月，低于低錶達者的36?5箇月（ P＜0?05）；但STOML2不同錶達水平患者的中位OS的差異無統計學意義（ P＞0?05）。結論 STOML2和TRIM25在HCC組織中呈高錶達，兩者聯閤檢測可用于評估肝癌的噁性錶現、分化情況及肝轉移程度，且TRIM25水平可能有助于生存預後的評估和預測。
목적：탐토간세포암（ HCC）조직중Stomatin양단백2（ STOML2）화삼기서단백25（ TRIM25）적표체수평급기여림상병리특정적관계。방법채용면역조화EnVision이보법검측75례HCC조직화61례암방정상간조직중STOML2화TRIM25적표체강도，근거세포내염색강도화양성세포수분급，분석STOML2、TRIM25적표체여HCC림상병리특정적관계급량자적상관성；동시채용Kaplan?Meier법분석STOML2、TRIM25불동표체강도적총생존기（ OS）。결과 STOML2화TRIM25재HCC조직중적고표체솔분별위68?0％（51／75）화64?0％（48／75），균고우암방조직적24?6％（15／61）화37?7％（23／61），차이유통계학의의（ P＜0?01）；STOML2표체여종류대소、분화정도、BCLC분기、문정맥암전화간외전이유관，이TRIM25단백표체여치료전갑태단백（AFP）、분화정도화복수균유관（P＜0?05），차STOML2여TRIM25표체정정상관（r＝0?379， P＝0?001）；TRIM25고표체자적중위OS위21?0개월，저우저표체자적36?5개월（ P＜0?05）；단STOML2불동표체수평환자적중위OS적차이무통계학의의（ P＞0?05）。결론 STOML2화TRIM25재HCC조직중정고표체，량자연합검측가용우평고간암적악성표현、분화정황급간전이정도，차TRIM25수평가능유조우생존예후적평고화예측。
Objective To explore the expressions of stomatin like protein 2 ( STOML2 ) and tripartite motif?containing 25 ( TRIM25) in the hepatocellular carcinoma( HCC) tissues as well as their relationship with clinicopathological characteristics. Methods The expressions of STOML2 and TRIM25 in 75 cases of HCC and 61 adjacent normal liver tissues were detected by immunohisto?chemical EnVision two?step method. The results were graded according to the intracellular staining intensity and the number of positive cells. Then the relationship of STOML2, TRIM25 expressions and the clinicopathological characteristics of HCC and the correlation of two proteins were analyzed. Meanwhile, the Kaplan?Meier survival curve was used to analyze the overall survival( OS) of HCC patients with different intensity of TRIM25 and STOML2. Results The over?expression rates of STOML2 and TRIM25 in HCC tissues were 68?0%(51/75) and 64?0%(48/75), both higher than 24?6%(15/61) and 37?7%(23/61) of the para?cancer tissues(P<0?01). The expression of STOML2 was related to tumor size, differentiation degree, BCLC staging, portal tumor thrombus and extrahepatic me?tastasis, while the expression of TRIM25 was associated with the AFP before treatment, differentiation degree and ascites(P<0?05). STOML2 was positively correlated with TRIM25( r=0?379,P=0?001) . The median OS of patients with high?level TRIM25 was 21?0 months, lower than 36?5 months of patients with low?level TRIM25(P<0?05), but there was no significant difference of median OS between patients with different levels of STOML2( P>0?05) . Conclusion STOML2 and TRIM25 are highly expressed in HCC tissues and the combined detection of STOML2 and TRIM25 should probably be helpful for the evaluation the malignancy and differentiation of HCC. TRIM25 may be useful for the assessment and prediction of prognosis of HCC.
