CAJ | 학술논문

目的：探讨非小细胞肺癌患者支气管肺泡灌洗液(BALF)中 RAR-β基因甲基化与 p53基因突变检测的临床意义及二者的相关性。方法收集非小细胞肺癌患者(肺癌组)85例及良性疾病患者(良性疾病组)70例的 BALF 标本,采用甲基化特异性 PCR(MSP)方法检测 BALF 中的 RAR-β基因甲基化,PCR 结合 DNA 测序法检测 p53基因突变。结果肺癌组 BALF 中RAR-β基因甲基化率及 p53基因突变率分别为49.4%、36.5%,均显著高于良性疾病组(P <0.01)；(Ⅰ+Ⅱ)期 RAR-β基因甲基化率(32.5%)高于同期 p53基因突变率(12.5%)(P <0.05)；(Ⅲ+Ⅳ)期 RAR-β基因甲基化率及 p53基因突变率均显著高于(Ⅰ+Ⅱ)期(P <0.01)；发生 RAR-β基因甲基化的肺癌患者 p53基因突变率更高(P <0.01)；发生 p53基因突变的肺癌患者RAR-β基因甲基化率更高(P <0.01)。结论检测非小细胞肺癌患者 BALF 中 RAR-β基因甲基化与 p53基因突变有助于肺癌的诊断。
목적：탐토비소세포폐암환자지기관폐포관세액(BALF)중 RAR-β기인갑기화여 p53기인돌변검측적림상의의급이자적상관성。방법수집비소세포폐암환자(폐암조)85례급량성질병환자(량성질병조)70례적 BALF 표본,채용갑기화특이성 PCR(MSP)방법검측 BALF 중적 RAR-β기인갑기화,PCR 결합 DNA 측서법검측 p53기인돌변。결과폐암조 BALF 중RAR-β기인갑기화솔급 p53기인돌변솔분별위49.4%、36.5%,균현저고우량성질병조(P <0.01)；(Ⅰ+Ⅱ)기 RAR-β기인갑기화솔(32.5%)고우동기 p53기인돌변솔(12.5%)(P <0.05)；(Ⅲ+Ⅳ)기 RAR-β기인갑기화솔급 p53기인돌변솔균현저고우(Ⅰ+Ⅱ)기(P <0.01)；발생 RAR-β기인갑기화적폐암환자 p53기인돌변솔경고(P <0.01)；발생 p53기인돌변적폐암환자RAR-β기인갑기화솔경고(P <0.01)。결론검측비소세포폐암환자 BALF 중 RAR-β기인갑기화여 p53기인돌변유조우폐암적진단。
Objective To investigate the clinical significance and correlation between RAR-βgene methylation and p53 gene mutation in bronchoalveolar lavage fluid(BALF)in non-small-cell lung cancer.Methods BALF samples from 85 lung cancer pa-tients(lung cancer group)and 70 cases(benign lung diseases group)with benign lung diseases were collected.RAR-βgene methyla-tion in BALF samples was detected by methylation-specific PCR (MSP),and p53 gene mutation was detected by PCR and DNA se-quencing method.Results The rate of RAR-βmethylation and p53 mutation in BALF in lung cancer were 49.4% and 36.5%,re-spectively.Both were higher than in benign lung diseases group(P <0.01).RAR-βmethylation rate(32.5%)of patients with TNM stages(Ⅰ+Ⅱ)(32.5%)was higher than the p53 mutation rate(12.5%)over the same stages (P <0.05).RAR-βmethylation rate and p53 mutation rate of patients with stages(Ⅲ+Ⅳ)were higher than those with stages(Ⅰ+Ⅱ)(P <0.01).p53 mutation rate in lung cancer patients with RAR-βmethylation was higher than those with unmethylated(P <0.01).RAR-βmethylation rate of lung cancer patients with p53 mutation was higher than those without p53 mutation(P <0.01).Conclusion Detection of RAR-βmethyl-ation and p53 mutation in BALF contribute to the diagnosis of lung cancer.