CAJ | 학술논문

目的：筛选出口腔鳞状细胞癌与颊黏膜癌前病变组织中的差异基因,并进行生物信息分析,探讨癌前病变转向鳞癌的分子机制。方法通过二羟甲基丁酸(DMBA)诱导金黄地鼠来建立颊黏膜癌前病变和鳞癌模型,提取病变组织总 RNA,合成单标 Cy3荧光标记的 cRNA,采用基因芯片技术,筛选出两组模型口腔组织中表达差异的基因,对筛选出的差异基因进行功能分类(GO)和信号通路(Pathway)分析,最后用 RT-PCR 验证其中部分差异基因。结果口腔鳞状细胞癌与颊黏膜癌前病变模型组织相比,有1981条基因差异表达(120条为未知基因),其中1037条为上调基因,944条为下调基因。GO 分析显示差异表达基因包括代谢、细胞结构、机体发育等14类相关的功能基因。通路分析结果显示有9条通路发生异常改变,在已知的1861条差异表达基因中,有14条基因富集于以上9条改变的通路上。结论颊黏膜癌前病变恶性转变到鳞癌共有1981条基因产生差异表达和9条通路发生异常改变,其中 Casp3和 CXCL12等14条基因参与了改变细胞通路,很可能就是癌前病变恶性转变成鳞癌的重要致病基因。
목적：사선출구강린상세포암여협점막암전병변조직중적차이기인,병진행생물신식분석,탐토암전병변전향린암적분자궤제。방법통과이간갑기정산(DMBA)유도금황지서래건립협점막암전병변화린암모형,제취병변조직총 RNA,합성단표 Cy3형광표기적 cRNA,채용기인심편기술,사선출량조모형구강조직중표체차이적기인,대사선출적차이기인진행공능분류(GO)화신호통로(Pathway)분석,최후용 RT-PCR 험증기중부분차이기인。결과구강린상세포암여협점막암전병변모형조직상비,유1981조기인차이표체(120조위미지기인),기중1037조위상조기인,944조위하조기인。GO 분석현시차이표체기인포괄대사、세포결구、궤체발육등14류상관적공능기인。통로분석결과현시유9조통로발생이상개변,재이지적1861조차이표체기인중,유14조기인부집우이상9조개변적통로상。결론협점막암전병변악성전변도린암공유1981조기인산생차이표체화9조통로발생이상개변,기중 Casp3화 CXCL12등14조기인삼여료개변세포통로,흔가능취시암전병변악성전변성린암적중요치병기인。
Objective To screen and analysis the virulent genes and pathways in golden hamster cheek pouch mucosa precan-cerous lesions and squamous cell carcinomas.Methods The experimental models of golden hamster cheek pouch mucosa precancer-ous lesions and squamous cell carcinomas were induced by DMBA.The total RNA of precancerous lesions and squamous cell carci-nomas of golden hamster cheek pouch was extracted and the cRNA was labeled by Cy3.Then gene chip was used to screen the dif-ferentially expressed genes.At last,the Gene Ontology and pathway was used to analysis the biology function of important virulent genes.Meanwhile,we confirmed the correctness of the results by using the RT-PCR.Results A total of 1 981 differentially ex-pressed genes were detected during the process from precancerous lesions to squamous cell carcinomas (120 genes remained known).One thousand and thirty-seven genes were up-regulated and 944 genes down-regulated.GO analysis showed that these dif-ferentially expressed genes mainly related to the macromolecular metabolism,signal transduction and so on.Pathway analysis showed that 9 pathways were significant changes.14 genes were enriched in above 9 change pathways.Conclusion There were 1 981 differentially expressed genes and 9 abnormal changes pathways during the process from precancerous lesions to squamous cell carcinomas,in which 14 differentially expressed genes led to changes in cellular pathways.These genes might be likely to have the important pathogenic genes in the process of transformation.