CAJ | 학술논문

以粘质沙雷氏菌 ZSG 为受试菌株，对灵菌红素分批发酵过程的溶氧和补料方式进行调控。分别以恒转速（200 r·min－1）与恒溶氧（10％、30％、50％）方式控制灵菌红素分批发酵过程，结果表明，与恒转速相比，恒溶氧控制方式能明显提高菌体干重，且在一定浓度范围内促进了灵菌红素的合成，在溶氧浓度为30％时，灵菌红素产量可提高69．0％。将溶氧浓度恒定在30％、维持最适 pH 值为6．7～7．8，通过流加补料，可使灵菌红素产量从补料前的79.01 mg ·L－1提高到115.98 mg·L－1，提高了46．8％。
이점질사뢰씨균 ZSG 위수시균주，대령균홍소분비발효과정적용양화보료방식진행조공。분별이항전속（200 r·min－1）여항용양（10％、30％、50％）방식공제령균홍소분비발효과정，결과표명，여항전속상비，항용양공제방식능명현제고균체간중，차재일정농도범위내촉진료령균홍소적합성，재용양농도위30％시，령균홍소산량가제고69．0％。장용양농도항정재30％、유지최괄 pH 치위6．7～7．8，통과류가보료，가사령균홍소산량종보료전적79.01 mg ·L－1제고도115.98 mg·L－1，제고료46．8％。
Dissolved oxygen(DO)concentration and feeding modes for prodigiosin(PG)batch fermentation were regulated using Serratia marcescens ZSG as testing strain.The process of PG batch fermentation under constant agitation rate of 200 r·min-1 and constant DO concentrations of 10%,30% and 50% were investiga-ted.Compared with constant agitation rate,constant DO concentrations significantly increased dry cell weight (DCW)and obviously promoted PG production within a reasonable range,PG production was improved by 69.0% under the constant DO concentration of 30%.Then the feeding mode of batch fermentation process was further studied under the constant DO concentration of 30%,optimum pH value of 6.7 ~ 7.8 and with flow feeding,the PG production was increased by 46.8% from 79.01 mg·L-1 to 115.98 mg·L-1 .