CAJ | 학술논문

通过富集培养，从被原油污染的土壤中筛选出一株嗜碱原油降解细菌 ODB04，对其进行鉴定及降解能力的研究。结果表明：该菌属肺炎克雷伯氏菌（Klebsiella pneumoniae ），其最佳生长条件为 NaCl 浓度1％～2％、温度35℃、pH 值8.0～9.0；在该条件下，200 r·min－1摇床振荡培养7 d，细菌 ODB04对原油的降解率为40％；在添加0.05％（体积分数）的非离子表面活性剂 Tween 80的情况下，其原油降解率高达56％，而0.05％（体积分数）非离子表面活性剂Triton X-100对细菌 ODB04的原油降解率影响不大。该菌对生物修复原油污染土壤具有一定的意义。
통과부집배양，종피원유오염적토양중사선출일주기감원유강해세균 ODB04，대기진행감정급강해능력적연구。결과표명：해균속폐염극뢰백씨균（Klebsiella pneumoniae ），기최가생장조건위 NaCl 농도1％～2％、온도35℃、pH 치8.0～9.0；재해조건하，200 r·min－1요상진탕배양7 d，세균 ODB04대원유적강해솔위40％；재첨가0.05％（체적분수）적비리자표면활성제 Tween 80적정황하，기원유강해솔고체56％，이0.05％（체적분수）비리자표면활성제Triton X-100대세균 ODB04적원유강해솔영향불대。해균대생물수복원유오염토양구유일정적의의。
A basophilic crude oil-degrading bacterium ODB04 was screened from crude oil-contaminated soil by enrichment cultivation.The strain ODB04 was identified as Klebsiella pneumoniae and its degradation capa-bility was evaluated.The optimal growth conditions for strain ODB04 were as follows:NaCl concentration 1%~2%,temperature 35 ℃ and pH value 8.0~9.0.The degradation rate of crude oil was 40% after incubation at 200 r·min-1 for 7 d under the optimal growth conditions and 56% with addition of 0.05%(volume fraction) non-ionic surfactant Tween 80.However,addition of 0.05%(volume fraction)non-ionic surfactant Triton X-100 had little effect on degradation rate.This bacterium is meaningful in bioremediation of crude oil-contaminated environment.