CAJ | 학술논문

以发光二极管为激发光源，以多模光纤代替透镜组作为激发光路，利用毛细管作为检测池，研制了一台小型、低功耗的荧光检测器。采用正交型光路设计，借助高精度机械加工的自校准平台，使光纤-毛细管-荧光收集透镜实现自校准。采用流动注射模式，以荧光素钠为标准样品对该检测器性能进行了测试，最低检测限为6．75×10－8 mol· L－1（S／N ＝3），在1．35×10－7～43．2×10－7 mol·L－1范围内线性关系良好（R 2＝0．9992），稳定性良好（RSD＜1％）。结合高效液相色谱系统，分离了异硫氰酸荧光素标记的4种氨基酸。
이발광이겁관위격발광원，이다모광섬대체투경조작위격발광로，이용모세관작위검측지，연제료일태소형、저공모적형광검측기。채용정교형광로설계，차조고정도궤계가공적자교준평태，사광섬-모세관-형광수집투경실현자교준。채용류동주사모식，이형광소납위표준양품대해검측기성능진행료측시，최저검측한위6．75×10－8 mol· L－1（S／N ＝3），재1．35×10－7～43．2×10－7 mol·L－1범위내선성관계량호（R 2＝0．9992），은정성량호（RSD＜1％）。결합고효액상색보계통，분리료이류청산형광소표기적4충안기산。
A small and low power efficient fluorescence detector was developed using light-emitting diode as excitation light source,multimode optical fiber as excitation light and capillary as testing pool.Based on orthog-onal optical arrangement,a homemade high precision machined platform was utilized in the self-alignment and self-calibration of optical fiber,capillary,and fluorescent collection lens.To evaluate the performance of the de-tector,a flow injection moldel was applied using sodium fluorescence as standard sample.Results showed that, the limit of detection(LOD)was 6.75×10-8 mol·L-1 (S/N =3),linear relationship was good in the range of 1.35×10-7 ~43.2×10-7 mol·L-1 (R 2 =0.9992)with good stability(RSD<1%).Four kinds of amino acids labeled by fluorescein isothiocyanate(FITC)were separated by the detector via HPLC method.