山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
31期
8-10
,共3页
马俊健%陈婷%刘彬%徐米清%XU Mi-qing
馬俊健%陳婷%劉彬%徐米清%XU Mi-qing
마준건%진정%류빈%서미청%XU Mi-qing
肾肿瘤%肾癌%细胞增殖%细胞凋亡%组蛋白去乙酰化酶抑制剂
腎腫瘤%腎癌%細胞增殖%細胞凋亡%組蛋白去乙酰化酶抑製劑
신종류%신암%세포증식%세포조망%조단백거을선화매억제제
kidney neoplasms%renal cell carcinoma%cell proliferation%apoptosis%histone deacetylase inhibitor
目的：观察LBH589对肾癌细胞株OS-RC-2细胞增殖的影响，并探讨其机制。方法将人肾癌细胞株OS-RC-2细胞分为DMSO组、N-乙酰半胱氨酸（NAC）组、LBH589组和NAC＋LBH589组，NAC组加入5 mmol／L NAC刺激2 h，LBH489组加入50 nmol／L LBH589刺激48 h，NAC＋LBH589组先加入5 mmol／L NAC预处理2 h，洗脱后再以50 nmol／L LBH589刺激48 h。 EdU法测算细胞增殖情况，流式细胞术检测细胞凋亡、活性氧簇（ ROS）， Western blotting法检测细胞p-STAT3、Cyclin D1蛋白。结果 DMSO组、NAC组、LBH589组、NAC＋LBH589组EdU染色阳性细胞比例分别为53％±4％、52％±5％、7％±3％、29％±6％，细胞凋亡率分别为0．25％±0．13％、0．18％±0．15％、11．35％±2．40％、1．92％±0．63％，ROS分别为0．60％±0．26％、0．33％±0．25％、12．2％±2．65％、5．33％±1．05％，DMSO组、NAC＋LBH589组与LBH589组比较，P均＜0．05。 DMSO组、NAC组、LBH589组、NAC＋LBH589组p-STAT3相对表达量分别为1．00±0．07、1．02±0．07、0．49±0．04、0．52±0．05，Cyclin D1相对表达量分别为1．00±0．08、1．03±0．08、0．45±0．04、0．49±0．03，DMSO组与LBH589组比较，P均＜0．05。结论LBH589对OS-RC-2肾癌细胞生长有抑制作用，其可能与诱导细胞凋亡、抑制细胞增殖、升高ROS 水平有关。LBH589能抑制STAT3信号通路，进而使Cyclin D1表达下降，而LBH589诱导ROS 升高不是通过抑制STAT3信号通路从而使肾癌细胞生长抑制。
目的：觀察LBH589對腎癌細胞株OS-RC-2細胞增殖的影響，併探討其機製。方法將人腎癌細胞株OS-RC-2細胞分為DMSO組、N-乙酰半胱氨痠（NAC）組、LBH589組和NAC＋LBH589組，NAC組加入5 mmol／L NAC刺激2 h，LBH489組加入50 nmol／L LBH589刺激48 h，NAC＋LBH589組先加入5 mmol／L NAC預處理2 h，洗脫後再以50 nmol／L LBH589刺激48 h。 EdU法測算細胞增殖情況，流式細胞術檢測細胞凋亡、活性氧簇（ ROS）， Western blotting法檢測細胞p-STAT3、Cyclin D1蛋白。結果 DMSO組、NAC組、LBH589組、NAC＋LBH589組EdU染色暘性細胞比例分彆為53％±4％、52％±5％、7％±3％、29％±6％，細胞凋亡率分彆為0．25％±0．13％、0．18％±0．15％、11．35％±2．40％、1．92％±0．63％，ROS分彆為0．60％±0．26％、0．33％±0．25％、12．2％±2．65％、5．33％±1．05％，DMSO組、NAC＋LBH589組與LBH589組比較，P均＜0．05。 DMSO組、NAC組、LBH589組、NAC＋LBH589組p-STAT3相對錶達量分彆為1．00±0．07、1．02±0．07、0．49±0．04、0．52±0．05，Cyclin D1相對錶達量分彆為1．00±0．08、1．03±0．08、0．45±0．04、0．49±0．03，DMSO組與LBH589組比較，P均＜0．05。結論LBH589對OS-RC-2腎癌細胞生長有抑製作用，其可能與誘導細胞凋亡、抑製細胞增殖、升高ROS 水平有關。LBH589能抑製STAT3信號通路，進而使Cyclin D1錶達下降，而LBH589誘導ROS 升高不是通過抑製STAT3信號通路從而使腎癌細胞生長抑製。
목적：관찰LBH589대신암세포주OS-RC-2세포증식적영향，병탐토기궤제。방법장인신암세포주OS-RC-2세포분위DMSO조、N-을선반광안산（NAC）조、LBH589조화NAC＋LBH589조，NAC조가입5 mmol／L NAC자격2 h，LBH489조가입50 nmol／L LBH589자격48 h，NAC＋LBH589조선가입5 mmol／L NAC예처리2 h，세탈후재이50 nmol／L LBH589자격48 h。 EdU법측산세포증식정황，류식세포술검측세포조망、활성양족（ ROS）， Western blotting법검측세포p-STAT3、Cyclin D1단백。결과 DMSO조、NAC조、LBH589조、NAC＋LBH589조EdU염색양성세포비례분별위53％±4％、52％±5％、7％±3％、29％±6％，세포조망솔분별위0．25％±0．13％、0．18％±0．15％、11．35％±2．40％、1．92％±0．63％，ROS분별위0．60％±0．26％、0．33％±0．25％、12．2％±2．65％、5．33％±1．05％，DMSO조、NAC＋LBH589조여LBH589조비교，P균＜0．05。 DMSO조、NAC조、LBH589조、NAC＋LBH589조p-STAT3상대표체량분별위1．00±0．07、1．02±0．07、0．49±0．04、0．52±0．05，Cyclin D1상대표체량분별위1．00±0．08、1．03±0．08、0．45±0．04、0．49±0．03，DMSO조여LBH589조비교，P균＜0．05。결론LBH589대OS-RC-2신암세포생장유억제작용，기가능여유도세포조망、억제세포증식、승고ROS 수평유관。LBH589능억제STAT3신호통로，진이사Cyclin D1표체하강，이LBH589유도ROS 승고불시통과억제STAT3신호통로종이사신암세포생장억제。
Objective To observe the influences of LBH589 on cell proliferation of renal cancer cell line OS-RC-2 and to explore its mechanism.Methods OS-RC-2 cells were divided into the DMSO group, N-acetyl cysteine ( NAC) group, LBH589 group and NAC+LBH589 group.NAC group was stimulated with 5 mmol/L of NAC for 2 h, LBH489 group was stimulated with 50 nmol/L of LBH589 for 48 h, and the NAC+LBH589 group was pre-treated with 5 mmol/L of NAC for 2 h and then after elution, the group was stimulated with 50 nmol/L of LBH589 for 48 h.EdU assay was used to measure cell proliferation, flow cytometry was used to detect the apoptosis and reactive oxygen species ( ROS) , while Western blotting was used to detect p-STAT3 and Cyclin D1 proteins.Results The proportions of positive Edu cells in the DMSO group, NAC group, LBH589 group and NAC+LBH589 group were respectively 53%±4%, 52%±5%, 7%±3%and 29%±6%.The apoptosis rates of the corresponding groups were 0.25%±0.13%, 0.18%±0.15%, 11.35%±2.40% and 1.92%± 0.63%.ROS of the corresponding groups were 0.60%±0.26%, 0.33%±0.25%, 12.2% ±2.65% and 5.33%± 1.05%.Significant difference was found between the DMSO, NAC+LBH589 groups and the LBH589 group (all P<0.05). The relative expression of p-STAT3 was 1.00 ±0.07, 1.02 ±0.07, 0.49 ±0.04 and 0.52 ±0.05 in the DMSO group, NAC group, LBH589 group and NAC+LBH589 group.The relative expression of Cyclin D1 was 1.00 ±0.08, 1.03 ±0.08, 0.45 ±0.04 and 0.49 ±0.03 in the corresponding groups.Significant difference was found between the DMSO group and the LBH589 group (all P<0.05).Conclusions LBH589 inhibits the growth of renal cancer cell line OS-RC-2, which may be related to induction of apoptosis, inhibition of cell proliferation and the increased ROS level.LBH589 can inhibit STAT3 sig-naling pathway, thus decrease the expression of Cyclin D1.However, LBH589-induced ROS increase is not achieved through the inhibition of STAT3 signaling pathway to inhibit the growth of renal cancer cells.
