山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
31期
14-16
,共3页
罗湘玉%罗卫民%张军%林称意%郭家龙
囉湘玉%囉衛民%張軍%林稱意%郭傢龍
라상옥%라위민%장군%림칭의%곽가룡
微小核糖核酸222%肺肿瘤%非小细胞肺癌%基质金属蛋白酶抑制剂3%细胞增殖%细胞侵袭
微小覈糖覈痠222%肺腫瘤%非小細胞肺癌%基質金屬蛋白酶抑製劑3%細胞增殖%細胞侵襲
미소핵당핵산222%폐종류%비소세포폐암%기질금속단백매억제제3%세포증식%세포침습
microRNA-222%lung neoplasm%non-small-cell lung cancer%matrix metalloproteinase inhibitor 3%cell proliferation%cell invasion
目的： miR-222基因转染对人非小细胞肺癌细胞增殖与侵袭的影响。方法采用qRT-PCR检测miR-222在A549、16HBE细胞中的表达；将miR-222 mimics、scramble、TIMP3-siRNA与si-control分别转染于A549细胞（设为实验1组、实验2组、实验3组、实验4组），Western blotting法检测细胞中的TIMP3蛋白，MTT法和Transwell侵袭实验分别检测细胞增殖与侵袭能力。结果 miR-222在A549、16HBE细胞的表达分别为0．767±0．086、0．405±0．034，两者比较，P＜0．01。实验1组、实验2组、实验3组、实验4组A549细胞中TIMP3蛋白表达分别为0．416±0．023、0．732±0．019、0．430±0．020与0．803±0．025，实验1组与实验2组比较，实验3组与实验4组比较，P均＜0．05。在A549细胞中过表达miR-222或沉默TIMP348、72、96 h后，细胞的增殖能力明显增强（ P均＜0．05）。实验1组、实验2组、实验3组、实验4组A549细胞48 h后穿过基质胶的细胞数分别为（104±5）、（204±8）、（99±4）、（188±7）个，实验1组与实验2组比较，实验3组与实验4组比较，P均＜0．05。结论 miR-222基因转染的人非小细胞肺癌细胞增殖与侵袭能力增强，其可能与miR-222靶向调控TIMP3作用有关。
目的： miR-222基因轉染對人非小細胞肺癌細胞增殖與侵襲的影響。方法採用qRT-PCR檢測miR-222在A549、16HBE細胞中的錶達；將miR-222 mimics、scramble、TIMP3-siRNA與si-control分彆轉染于A549細胞（設為實驗1組、實驗2組、實驗3組、實驗4組），Western blotting法檢測細胞中的TIMP3蛋白，MTT法和Transwell侵襲實驗分彆檢測細胞增殖與侵襲能力。結果 miR-222在A549、16HBE細胞的錶達分彆為0．767±0．086、0．405±0．034，兩者比較，P＜0．01。實驗1組、實驗2組、實驗3組、實驗4組A549細胞中TIMP3蛋白錶達分彆為0．416±0．023、0．732±0．019、0．430±0．020與0．803±0．025，實驗1組與實驗2組比較，實驗3組與實驗4組比較，P均＜0．05。在A549細胞中過錶達miR-222或沉默TIMP348、72、96 h後，細胞的增殖能力明顯增彊（ P均＜0．05）。實驗1組、實驗2組、實驗3組、實驗4組A549細胞48 h後穿過基質膠的細胞數分彆為（104±5）、（204±8）、（99±4）、（188±7）箇，實驗1組與實驗2組比較，實驗3組與實驗4組比較，P均＜0．05。結論 miR-222基因轉染的人非小細胞肺癌細胞增殖與侵襲能力增彊，其可能與miR-222靶嚮調控TIMP3作用有關。
목적： miR-222기인전염대인비소세포폐암세포증식여침습적영향。방법채용qRT-PCR검측miR-222재A549、16HBE세포중적표체；장miR-222 mimics、scramble、TIMP3-siRNA여si-control분별전염우A549세포（설위실험1조、실험2조、실험3조、실험4조），Western blotting법검측세포중적TIMP3단백，MTT법화Transwell침습실험분별검측세포증식여침습능력。결과 miR-222재A549、16HBE세포적표체분별위0．767±0．086、0．405±0．034，량자비교，P＜0．01。실험1조、실험2조、실험3조、실험4조A549세포중TIMP3단백표체분별위0．416±0．023、0．732±0．019、0．430±0．020여0．803±0．025，실험1조여실험2조비교，실험3조여실험4조비교，P균＜0．05。재A549세포중과표체miR-222혹침묵TIMP348、72、96 h후，세포적증식능력명현증강（ P균＜0．05）。실험1조、실험2조、실험3조、실험4조A549세포48 h후천과기질효적세포수분별위（104±5）、（204±8）、（99±4）、（188±7）개，실험1조여실험2조비교，실험3조여실험4조비교，P균＜0．05。결론 miR-222기인전염적인비소세포폐암세포증식여침습능력증강，기가능여miR-222파향조공TIMP3작용유관。
Objective To investigate the effects of miR-222 gene transfection on proliferation and invasion of human non-small-cell lung cancer cells.Methods The qRT-PCR was employed to detect the expression of miR-222 in A549 and 16HBE cells.The miR-222 mimics, scramble, TIMP3-siRNA and si-control sequence were transfected into A549 cells ( experimental group 1, experimental group 2, experimental group 3 and experimental group 4), respectively.Western blotting was used to de-tect the expression of TIMP3 protein.The proliferation and invasion abilities were evaluated by MTT and Transwell invasion as-say.Results The expression of miR-222 in A549 cells and 16HBE cells was respectively 0.767 ±0.086 and 0.405 ±0.034 (P<0.01).The expression levels of TIMP3 protein in the A549 cells of the experimental group 1, experimental group 2, experi-mental group 3 and experimental group 4 were 0.416 ±0.023, 0.732 ±0.019, 0.430 ±0.020 and 0.803 ±0.025, respectively. Significant difference was found between the experimental groups 1 and 2, between the experimental groups 3 and 4 ( all P<0.05).The cell proliferation ability was increased in A549 cells after overexpressing miR-222 and silencing TIMP3 for 48 h, 72 h and 96 h (all P<0.05).After 48 h, in the A549 cells, the number of cells through the basement membrane in the experimen-tal group 1, experimental group 2, experimental group 3 and experimental group 4 were 104 ±5, 204 ±8, 99 ±4 and 188 ±7, re-spectively.Significant difference was found between the experimental groups 1 and 2, between the experimental groups 3 and 4 (all P<0.05).Conclusion The proliferation and invasion abilities of human non-small-cell lung cancer cells transfected by miR-222 are enhanced which may be related with miR-222 targeted-regulating TIMP3.
