中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2015年
22期
93-95,98
,共4页
蒋德菊%刘美霞%陈绍玲%李康
蔣德菊%劉美霞%陳紹玲%李康
장덕국%류미하%진소령%리강
复发性流产%MMP-9%TIMP-1
複髮性流產%MMP-9%TIMP-1
복발성유산%MMP-9%TIMP-1
Recurrent spontaneous abortion%MMP-9%TIMP-1
目的：检测MMP-9、TIMP-1在复发性流产绒毛组织中的mRNA相对表达量,研究MMP-9和TIMP-1的表达失衡与复发性流产的相关性。方法选取2013年4~12月我院妇科门诊正常早孕和复发性流产妇女的绒毛组织各19例和28例,RT-PCR检测MMP-9、TIMP-1的相对表达量。结果复发性流产组绒毛MMP-9 mRNA的相对表达量为4.11±0.42,正常早孕组绒毛MMP-9 mRNA的相对表达量为2.45±0.36,两组比较差异有统计学意义(P<0.01)；复发性流产组绒毛TIMP-1 mRNA相对表达量为7.62±1.54,正常早孕组绒毛TIMP-1相对表达量为8.41±1.02,两组比较,差异无统计学意义(P>0.05)。结论复发性流产绒毛组织中的MMP-9 mRNA表达量显著下降,TIMP-1 mRNA表达量升高,MMP-9和TIMP-1的表达失衡参与了复发性流产的发生、发展。
目的：檢測MMP-9、TIMP-1在複髮性流產絨毛組織中的mRNA相對錶達量,研究MMP-9和TIMP-1的錶達失衡與複髮性流產的相關性。方法選取2013年4~12月我院婦科門診正常早孕和複髮性流產婦女的絨毛組織各19例和28例,RT-PCR檢測MMP-9、TIMP-1的相對錶達量。結果複髮性流產組絨毛MMP-9 mRNA的相對錶達量為4.11±0.42,正常早孕組絨毛MMP-9 mRNA的相對錶達量為2.45±0.36,兩組比較差異有統計學意義(P<0.01)；複髮性流產組絨毛TIMP-1 mRNA相對錶達量為7.62±1.54,正常早孕組絨毛TIMP-1相對錶達量為8.41±1.02,兩組比較,差異無統計學意義(P>0.05)。結論複髮性流產絨毛組織中的MMP-9 mRNA錶達量顯著下降,TIMP-1 mRNA錶達量升高,MMP-9和TIMP-1的錶達失衡參與瞭複髮性流產的髮生、髮展。
목적：검측MMP-9、TIMP-1재복발성유산융모조직중적mRNA상대표체량,연구MMP-9화TIMP-1적표체실형여복발성유산적상관성。방법선취2013년4~12월아원부과문진정상조잉화복발성유산부녀적융모조직각19례화28례,RT-PCR검측MMP-9、TIMP-1적상대표체량。결과복발성유산조융모MMP-9 mRNA적상대표체량위4.11±0.42,정상조잉조융모MMP-9 mRNA적상대표체량위2.45±0.36,량조비교차이유통계학의의(P<0.01)；복발성유산조융모TIMP-1 mRNA상대표체량위7.62±1.54,정상조잉조융모TIMP-1상대표체량위8.41±1.02,량조비교,차이무통계학의의(P>0.05)。결론복발성유산융모조직중적MMP-9 mRNA표체량현저하강,TIMP-1 mRNA표체량승고,MMP-9화TIMP-1적표체실형삼여료복발성유산적발생、발전。
Objective To detect the relative expression of MMP-9,TIMP-1 in recurrent spontaneous abortion villus tis-sues of mRNA,to study the correlation of MMP-9 and TIMP-1 expression imbalance and recurrent spontaneous abor-tion. Methods Normal early pregnancy (19 cases)and recurrent spontaneous abortion (28 cases) villus tissues in our hospital from April to December 2013 were selected.The relative expression of RT-PCR was detected in MMP-9, TIMP-1. Results The recurrent spontaneous abortion group of relative expression of MMP-9 mRNA was 4.11+0.42, normal pregnancy group of the relative expression of MMP-9 mRNA was 2.45+0.36,to be compared with the normal group,the expression was significantly decreased,there was significant difference(P<0.01).Recurrent spontaneous abortion group of the relative expression of TIMP-1 mRNA was 7.62+1.54,normal group of the relative expression of TIMP-1 was 8.41+1.02,to be compared with the normal group,the expression was increased,but the difference was not significant (P>0.05). Conclusion In recurrent spontaneous abortion villus tissues,the expression of MMP-9 mRNA significantly decreases,the expression of TIMP-1 mRNA increases,the unbalanced expression of MMP-9 and TIMP-1 takes part in the occurrence and development of recurrent spontaneous abortion.
