CAJ | 학술논문

目的：比较超敏HBV DNA检测系统与传统荧光定量PCR外标法定量检测血清乙肝病毒DNA，探讨其临床应用价值。方法：采用超敏PCR检测系统和传统荧光定量PCR外标法同时检测80例HBsAg阳性患者的血清HBV DNA浓度。结果：超敏法阳性检出率(92.5%)高于传统外标法(50.0%)(P<0.01)。超敏法阳性定量结果中位值为2.95×105 IU/mL，高于传统外标法(5.63×104 IU/mL)(P<0.01)，两种方法对高载量病毒(≥104 IU/mL)血清标本检测结果的相关性有统计学意义(R2=0.89，P<0.01)。超敏法对26例HBeAg阴性慢性乙肝患者的阳性检出率(100.0%)高于传统外标法(11.5%)(P<0.01)。结论：超敏HBV DNA定量检测系统检测灵敏度高，更适用于临床低载量病毒(<104 IU/mL)血清标本的检测。
목적：비교초민HBV DNA검측계통여전통형광정량PCR외표법정량검측혈청을간병독DNA，탐토기림상응용개치。방법：채용초민PCR검측계통화전통형광정량PCR외표법동시검측80례HBsAg양성환자적혈청HBV DNA농도。결과：초민법양성검출솔(92.5%)고우전통외표법(50.0%)(P<0.01)。초민법양성정량결과중위치위2.95×105 IU/mL，고우전통외표법(5.63×104 IU/mL)(P<0.01)，량충방법대고재량병독(≥104 IU/mL)혈청표본검측결과적상관성유통계학의의(R2=0.89，P<0.01)。초민법대26례HBeAg음성만성을간환자적양성검출솔(100.0%)고우전통외표법(11.5%)(P<0.01)。결론：초민HBV DNA정량검측계통검측령민도고，경괄용우림상저재량병독(<104 IU/mL)혈청표본적검측。
Objective: To compare a highly sensitive detection system with a traditional real-time PCR assay with external standards for HBV DNA quantiifcation.Methods: hTe concentrations of serum HBV DNA in 80 HBsAg-positive patients were determined by both a highly sensitive detection system and a traditional real-time PCR assay with external standards. Results: hTe positive rate determined by the highly sensitive system (92.5%) was signiifcantly higher than that by the traditional assay (50.0%) (P<0.01). The median HBV DNA concentration quantified by the highly sensitive system was 2.95×105 IU/mL, which was signiifcantly higher than that by the traditional assay (5.63×104 IU/mL) (P<0.01). hTere was a high correlation between HBV DNA concentrations (≥104 IU/mL) determined by the two different assays (R2=0.89,P<0.01). hTe positive rate of 26 HBeAg-negative patients determined by highly sensitive system (100.0%) was signiifcantly higher than that by traditional assay (11.5%) (P<0.01).Conclusion: hTe highly sensitive system is more suitable for HBV DNA quantiifcation of clinical serum samples with low virus load (<104 IU/mL).