CAJ | 학술논문

目的：研究一种新的国家一类中药金天格胶囊对骨质疏松性骨折大鼠骨折愈合过程中细胞因子的影响。方法建立绝经后骨质疏松性骨折大鼠模型，给予雌激素及不同浓度的金天格胶囊灌胃，并于灌胃治疗5周后，采用酶联免疫吸附法（ELISA）测定白细胞介素－2（IL－2）、白细胞介素－4（IL－4）及肿瘤坏死因子－α（TNF－α）的表达。结果模型组大鼠的IL－2、IL－4的阳性表达明显降低、TNF－α的阳性表达明显升高（P＜0．05）；中、低剂量的金天格胶囊可使大鼠的IL－2、IL－4的阳性表达明显升高、TNF－α的阳性表达明显降低（P＜0．05），而高剂量金天格胶囊的干预效果不显著（P＞0．05）。结论①IL－2、IL－4是促进骨质疏松性骨折愈合的细胞因子，与TNF－α的作用相反②金天格胶囊对骨质疏松性骨折大鼠的愈合具有促进作用。
목적：연구일충신적국가일류중약금천격효낭대골질소송성골절대서골절유합과정중세포인자적영향。방법건립절경후골질소송성골절대서모형，급여자격소급불동농도적금천격효낭관위，병우관위치료5주후，채용매련면역흡부법（ELISA）측정백세포개소－2（IL－2）、백세포개소－4（IL－4）급종류배사인자－α（TNF－α）적표체。결과모형조대서적IL－2、IL－4적양성표체명현강저、TNF－α적양성표체명현승고（P＜0．05）；중、저제량적금천격효낭가사대서적IL－2、IL－4적양성표체명현승고、TNF－α적양성표체명현강저（P＜0．05），이고제량금천격효낭적간예효과불현저（P＞0．05）。결론①IL－2、IL－4시촉진골질소송성골절유합적세포인자，여TNF－α적작용상반②금천격효낭대골질소송성골절대서적유합구유촉진작용。
Objective To study the effects of a class of the new state traditional Chinese medicine, Jintiange capsules, on the expression of cytokines during the fracture healing process in osteoporotic rats.Methods The rat model of postmenopausal osteoporotic fracture was established.Jintiange capsules and different concentrations of estrogen were administered through stomach. After 5 weeks of the treatment, expression of interleukin-2 (IL-2), interleukin-4 (IL-4), and tumor necrosis factor alpha (TNF-α) were determined using enzyme-linked immunosorbent assay ( ELISA ) .Results The positive expression of IL-2 and IL-4 decreased significantly, but the expression of TNF-αincreased significantly in rats of model group ( P<0.05 ) .Low and middle doses of Jintiange capsules increased the positive expression of IL-2 and IL-4, but decreased TNF-αsignificantly in rat (P<0.05). No significant intervention with high dose of Jintiange capsules was found (P>0.05).Conclusion 1) IL-2 and IL-4 are cytokines that promote the healing of osteoporotic fractures, which is opposite to the effect of TNF-α.2 ) Jintiange capsules can promote the healing of osteoporotic fractures.