CAJ | 학술논문

目的：探讨建立操作简便可行、成功率高、具有良好模拟性的新生大鼠高胆红素血症动物型，为开展高胆红素血症致核黄疸及相关神经损伤机制研究提供实验基础模型。方法随机选取7日龄SD大鼠，分别采用25、50、75 mg／kg三个梯度腹腔内注射盐酸苯肼，建立溶血致高胆红素血症动物模型，同时设立对照组，分别在实验处理48h后，测定血、脑组织胆红素、同时进行脑组织NSE分析对模型进行评价。结果三组实验组分别与对照组相比，血、脑组织胆红素、脑组织NSE均大于对照组，差异具有显著性（ P＜0.05），而血红蛋白含量小于对照组，差异具有显著性（ P＜0.05）；50 mg和75 mg剂量组血、脑组织胆红素、脑组织神经元特异性烯醇化酶（ NSE）均大于25 mg剂量组，而血红蛋白含量小于25 mg剂量组，差异具有显著性（P＜0.05）；50 mg和75 mg剂量组间相比各项指标差异无显著性（ P＞0.05）。结论腹腔注射盐酸苯肼能够制作出符合临床特征的高胆红素动物模型，50 mg／kg为最佳浓度，是建立新生儿高胆红素血症动物模型的理想方法。
목적：탐토건립조작간편가행、성공솔고、구유량호모의성적신생대서고담홍소혈증동물형，위개전고담홍소혈증치핵황달급상관신경손상궤제연구제공실험기출모형。방법수궤선취7일령SD대서，분별채용25、50、75 mg／kg삼개제도복강내주사염산분정，건립용혈치고담홍소혈증동물모형，동시설립대조조，분별재실험처리48h후，측정혈、뇌조직담홍소、동시진행뇌조직NSE분석대모형진행평개。결과삼조실험조분별여대조조상비，혈、뇌조직담홍소、뇌조직NSE균대우대조조，차이구유현저성（ P＜0.05），이혈홍단백함량소우대조조，차이구유현저성（ P＜0.05）；50 mg화75 mg제량조혈、뇌조직담홍소、뇌조직신경원특이성희순화매（ NSE）균대우25 mg제량조，이혈홍단백함량소우25 mg제량조，차이구유현저성（P＜0.05）；50 mg화75 mg제량조간상비각항지표차이무현저성（ P＞0.05）。결론복강주사염산분정능구제작출부합림상특정적고담홍소동물모형，50 mg／kg위최가농도，시건립신생인고담홍소혈증동물모형적이상방법。
Objective To establish and evaluate a reliable and highly reproducible neonatal rat model of hyper-bilirubinemia and to provide an experimental basis for research of kernicterus and related mechanism of neuroinjury.Meth-ods Sixty 7-day old SD rats (28 male and 32 female) were used in this study.Three doses of phenylhydrazine hydrochlo-ride (25, 50, and 75 mg/kg) were intraperitoneally injected respectively to the neonatal rats to establish models of hyper-bilirubinemia induced by hemolysis.The control group was set up at the same time.48 hours after the experimental treat-ment, the bilirubin in blood and brain tissue, neuron-specific enolase (NSE) of brain tissue, and hemoglobin were detec-ted to evaluate the models.Results Compared with the control group, the bilirubin in the blood and brain tissue and the brain tissue NSE in the three experimental groups were significantly higher than that in the control group (P<0.05), while hemoglobin content was significantly lower than that in the control group (P<0.05).The bilirubin of blood and brain tis-sue and brain tissue NSE in the 50 mg/kg and 75 mg/kg dose phenylhydrazine hydrochloride groups were significantly high-er than that of the 25 mg/kg dose group ( P<0.05) , while hemoglobin content was significantly lower than that of the 25 mg/kg dose group ( P<0.05 ) .There were no significant differences between the 50 mg and 75 mg dose groups ( P>0.05).Conclusions Intraperitoneal injection of phenylhydrazine hydrochloride can be used to produce neonatal rat mod-els of hyperbilirubinemia, mimicking the clinical features of this disease, and 50 mg/kg of phenylhydrazine hydrochloride is the best concentration.It is an ideal method to establish newborn rat models of hyperbilirubinemia.