CAJ | 학술논문

利用 A 值法与活菌平板计数法,测定了杀鲑气单胞菌在不同条件下的生长曲线,研究了不同温度及pH对杀鲑气单胞菌(Aeromonas salmonicha)生长的影响。结果表明,活菌计数法更能真实的反映细菌数量的动态变化。在28℃,15℃,10℃,5℃条件下,随着温度降低,杀鲑气单胞菌生长速率明显下降；在pH6.0~8.0时,酸性条件能明显抑制杀鲑气单胞菌的生长；在28℃、pH7.5条件下,杀鲑气单胞菌生长速率最快,达到稳定期时细菌数量也最多。在养殖生产过程中,为了抑制杀鲑气单胞菌的生长繁殖,建议在不影响鱼类正常生长的条件下,尽量降低养殖水体温度并合理控制水体pH,以降低养殖鱼类发病率。
이용 A 치법여활균평판계수법,측정료살해기단포균재불동조건하적생장곡선,연구료불동온도급pH대살해기단포균(Aeromonas salmonicha)생장적영향。결과표명,활균계수법경능진실적반영세균수량적동태변화。재28℃,15℃,10℃,5℃조건하,수착온도강저,살해기단포균생장속솔명현하강；재pH6.0~8.0시,산성조건능명현억제살해기단포균적생장；재28℃、pH7.5조건하,살해기단포균생장속솔최쾌,체도은정기시세균수량야최다。재양식생산과정중,위료억제살해기단포균적생장번식,건의재불영향어류정상생장적조건하,진량강저양식수체온도병합리공제수체pH,이강저양식어류발병솔。
In this paper, the effects of different temperatures and pH on the growth ofAeromonas salmonicida were studied. The results showed that plate count method could better reflect the true dynamic growth condition ofA. salmonicida. At the four temperature gradients (28, 15, 10 and 5℃), as the temperature decreased, thegrowth rate of A. salmonicida decreased obviously. Within the range of pH 6.0~8.0,A. salmonicida could grow, but its growth was significantly inhibited under acid conditions. The results also showed thatA. salmonicida had gained the high-est growth rate and the largest amount under the condition of 28℃ and pH 7.5. This study has a certain theoretical guiding significance to the actual production. In order to inhibit the growth ofA. salmonicida and reduce the infec-tion of the farmed fish,it is strongly suggested to reduce the temperature and control the pH value in aquaculture reasonably.