CAJ | 학술논문

为探讨外源核黄素对盐胁迫下杜梨叶片抗氧化系统的影响，以梨砧木杜梨幼苗为供试材料，在水培条件下，研究了外源施加不同浓度核黄素对200 mmol/L NaCl胁迫下其叶片抗氧化酶活性、活性氧产生、膜质过氧化和抗氧化物质含量的影响。结果显示：NaCl胁迫3 d后，杜梨叶片中超氧化物歧化酶( SOD)活性减弱，过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、抗坏血酸过氧化物酶(APX)的活性增强，抗氧化物质谷胱甘肽(GSH)和抗坏血酸(AsA)合成下降，活性氧(O2·-、H2O2)和丙二醛(MDA)大量积累。施加外源核黄素能增强NaCl胁迫下杜梨叶片中SOD、POD、CAT、GR、GSH-Px和APX的活性，提高GSH和AsA的含量，减少O2·-和H2 O2的产生，降低脂质过氧化程度，有效缓解盐胁迫对杜梨叶片的过氧化伤害，其中以10μmol/L浓度的核黄素处理效果最为显著。
위탐토외원핵황소대염협박하두리협편항양화계통적영향，이리침목두리유묘위공시재료，재수배조건하，연구료외원시가불동농도핵황소대200 mmol/L NaCl협박하기협편항양화매활성、활성양산생、막질과양화화항양화물질함량적영향。결과현시：NaCl협박3 d후，두리협편중초양화물기화매( SOD)활성감약，과양화물매(POD)、과양화경매(CAT)、곡광감태환원매(GR)、곡광감태과양화물매(GSH-Px)、항배혈산과양화물매(APX)적활성증강，항양화물질곡광감태(GSH)화항배혈산(AsA)합성하강，활성양(O2·-、H2O2)화병이철(MDA)대량적루。시가외원핵황소능증강NaCl협박하두리협편중SOD、POD、CAT、GR、GSH-Px화APX적활성，제고GSH화AsA적함량，감소O2·-화H2 O2적산생，강저지질과양화정도，유효완해염협박대두리협편적과양화상해，기중이10μmol/L농도적핵황소처리효과최위현저。
To explore the effect of exogenous riboflavin on the antioxidant system in the leaves of Pyrus betulaefolia un-der salt stress, the antioxidant enzymes activities, the production of active oxygen, lipid peroxidation and antioxidants con-tents of the leaves of P. betulaefolia were analysed when its seedlings under 200 mmol/L NaCl stress were exposed to different concentrations (0 μmol/L, 5μmol/L, 10μmol/L, 50μmol/L, 100μmol/L) of riboflavin in hydroponic culture, after 3-d salt stress, the activity of superoxide dismutase ( SOD) was decreased, the activities of peroxidase ( POD) , catalase ( CAT) , glutathione reductase ( GR) , glutathione peroxidase ( GSH-Px) and ascorbic acid peroxidase ( APX) were enhanced, the con-tents of GSH and AsA were reduced, and the accumulation of O2·-, H2 O2 and MDA were increased. Riboflavin application al-leviated the damage caused by NaCl by improving the activities of SOD, POD, CAT, GR, GSH-Px and APX, increasing the contents of GSH and AsA, reducing the productions of O2 ·- and H2 O2 were decreased, and decreasing the degree of lipid peroxidation. The riboflavin with the concentration of 10μmol/L showed the best effects.