CAJ | 학술논문

本试验建立了一种无需衍生反应即可实现高效液相色谱搭载可变波长检测器( HPLC-VWD)直接检测桃果肉中蔗糖、葡萄糖、果糖和山梨醇含量的方法，并对该方法进行了评价。对HPLC-VWD和高效液相色谱搭载示差折光检测器( HPLC-RID)测得不同桃品种中糖组分进行比较分析；并利用建立的HPLC-VWD法测定了葡萄、苹果、粉蕉和梨果肉中糖组分。结果显示，本研究建立的HPLC-VWD法可对糖组分进行有效分离和检测，在相应的范围内线性关系良好，相对标准偏差为0.53%~2.71%，回收率为92%~103%；HPLC-VWD和HPLC-RID法测得结果没有显著差异。
본시험건립료일충무수연생반응즉가실현고효액상색보탑재가변파장검측기( HPLC-VWD)직접검측도과육중자당、포도당、과당화산리순함량적방법，병대해방법진행료평개。대HPLC-VWD화고효액상색보탑재시차절광검측기( HPLC-RID)측득불동도품충중당조분진행비교분석；병이용건립적HPLC-VWD법측정료포도、평과、분초화리과육중당조분。결과현시，본연구건립적HPLC-VWD법가대당조분진행유효분리화검측，재상응적범위내선성관계량호，상대표준편차위0.53%~2.71%，회수솔위92%~103%；HPLC-VWD화HPLC-RID법측득결과몰유현저차이。
A reliable analysis system of high-performance liquid chromatography with variable wavelength detector ( HPLC-VWD) for detecting sugars in peach, including sucrose, glucose, fructose and sorbitol, were established and eval-uated. It could separate and detect sugars in the flesh of fruits effectively. A linear relationship was concluded between peak area and sugar concentration, with relative standard deviation (RSD) for the accuracy of 0. 53%-2. 71% and the re-covery of 92%-103%. There was no significant difference between the sugar contents in different peach cultivars detected by HPLC-VWD and high performance liquid chromatography with refractive index detector(HPLC-RID). The sugars con-tents in grape, apple, banana and pear detected by HPLC-VWD were the same as those detected in other researches. The analysis system of HPLC-VWD without derivation was reliable for the determination of sugars in fruits.