华中科技大学学报(医学版)
華中科技大學學報(醫學版)
화중과기대학학보(의학판)
ACTA UNIVERSITATIS MEDICINAE TONGJI
2015年
4期
419-423
,共5页
张智星%冯祥礼%毛靖%肖建中%邱进俊%刘承美
張智星%馮祥禮%毛靖%肖建中%邱進俊%劉承美
장지성%풍상례%모정%초건중%구진준%류승미
β-磷酸三钙%不饱和聚磷酸酯%骨缺损%骨修复%吸收率
β-燐痠三鈣%不飽和聚燐痠酯%骨缺損%骨脩複%吸收率
β-린산삼개%불포화취린산지%골결손%골수복%흡수솔
β-tricalcium phosphate%unsaturated polyphosphoester%bone defects%bone repair%resorption ratio
目的:研究自制的可注射骨修复材料植入兔股骨缺损区后的骨组织反应特点。方法选用25只新西兰大白兔,在其双侧股骨内髁制作标准骨缺损区模型,分别注射不饱和聚磷酸酯/β‐磷酸三钙复合物(UPPE/β‐TCP)和添加1%四环素的UPPE/β‐TCP复合物(UPPE/β‐TCP/TTC),按2、4、8、12、24周的植入期分为5组,对骨组织样本进行组织病理学和形态学观察,并比较各期植入体的吸收率。结果在各植入期内,UPPE/β‐TCP和UPPE/β‐TCP/TTC两种复合物的骨组织反应基本一致。植入2周时,所有材料和周围骨组织均未发生明显的吸收或改建,植入体未引起周围骨组织的炎性反应和坏死;4周时植入体与骨缺损区结合良好,无纤维结缔组织囊形成,在材料发生吸收而改建的区域,可见成骨细胞、新生血管和活跃的新骨形成;8周时大部分植入体仍存在于骨缺损区内,部分植入体成为“独岛”被完全整合进入新生的骨组织中;12周时骨组织有广泛的重建;24周时植入体主体部分消失,骨组织内的生长和改建更加活跃,新生骨和骨髓样组织中可见大量材料碎片,并可见成骨样细胞。2种复合物植入骨缺损区后的吸收率均随植入时间的延长而逐渐增加,在8周以前其吸收率相似(P>0.05),而12周时UPPE/β‐TCP/TTC复合物组的吸收率高于UPPE/β‐TCP复合物(P<0.05),24周时2种复合物的吸收率均在60%以上。结论可注射骨修复材料UPPE/β‐TCP复合物在动物体内具有良好的骨组织生物相容性、生物可降解性和体内骨引导性,可作为修复骨缺损的新型复合材料。
目的:研究自製的可註射骨脩複材料植入兔股骨缺損區後的骨組織反應特點。方法選用25隻新西蘭大白兔,在其雙側股骨內髁製作標準骨缺損區模型,分彆註射不飽和聚燐痠酯/β‐燐痠三鈣複閤物(UPPE/β‐TCP)和添加1%四環素的UPPE/β‐TCP複閤物(UPPE/β‐TCP/TTC),按2、4、8、12、24週的植入期分為5組,對骨組織樣本進行組織病理學和形態學觀察,併比較各期植入體的吸收率。結果在各植入期內,UPPE/β‐TCP和UPPE/β‐TCP/TTC兩種複閤物的骨組織反應基本一緻。植入2週時,所有材料和週圍骨組織均未髮生明顯的吸收或改建,植入體未引起週圍骨組織的炎性反應和壞死;4週時植入體與骨缺損區結閤良好,無纖維結締組織囊形成,在材料髮生吸收而改建的區域,可見成骨細胞、新生血管和活躍的新骨形成;8週時大部分植入體仍存在于骨缺損區內,部分植入體成為“獨島”被完全整閤進入新生的骨組織中;12週時骨組織有廣汎的重建;24週時植入體主體部分消失,骨組織內的生長和改建更加活躍,新生骨和骨髓樣組織中可見大量材料碎片,併可見成骨樣細胞。2種複閤物植入骨缺損區後的吸收率均隨植入時間的延長而逐漸增加,在8週以前其吸收率相似(P>0.05),而12週時UPPE/β‐TCP/TTC複閤物組的吸收率高于UPPE/β‐TCP複閤物(P<0.05),24週時2種複閤物的吸收率均在60%以上。結論可註射骨脩複材料UPPE/β‐TCP複閤物在動物體內具有良好的骨組織生物相容性、生物可降解性和體內骨引導性,可作為脩複骨缺損的新型複閤材料。
목적:연구자제적가주사골수복재료식입토고골결손구후적골조직반응특점。방법선용25지신서란대백토,재기쌍측고골내과제작표준골결손구모형,분별주사불포화취린산지/β‐린산삼개복합물(UPPE/β‐TCP)화첨가1%사배소적UPPE/β‐TCP복합물(UPPE/β‐TCP/TTC),안2、4、8、12、24주적식입기분위5조,대골조직양본진행조직병이학화형태학관찰,병비교각기식입체적흡수솔。결과재각식입기내,UPPE/β‐TCP화UPPE/β‐TCP/TTC량충복합물적골조직반응기본일치。식입2주시,소유재료화주위골조직균미발생명현적흡수혹개건,식입체미인기주위골조직적염성반응화배사;4주시식입체여골결손구결합량호,무섬유결체조직낭형성,재재료발생흡수이개건적구역,가견성골세포、신생혈관화활약적신골형성;8주시대부분식입체잉존재우골결손구내,부분식입체성위“독도”피완전정합진입신생적골조직중;12주시골조직유엄범적중건;24주시식입체주체부분소실,골조직내적생장화개건경가활약,신생골화골수양조직중가견대량재료쇄편,병가견성골양세포。2충복합물식입골결손구후적흡수솔균수식입시간적연장이축점증가,재8주이전기흡수솔상사(P>0.05),이12주시UPPE/β‐TCP/TTC복합물조적흡수솔고우UPPE/β‐TCP복합물(P<0.05),24주시2충복합물적흡수솔균재60%이상。결론가주사골수복재료UPPE/β‐TCP복합물재동물체내구유량호적골조직생물상용성、생물가강해성화체내골인도성,가작위수복골결손적신형복합재료。
Objective To assess in vivo bone tissue response to an injectable bone substitute after implantation using a rab‐bit model.Methods Twenty‐five male New Zealand white rabbits were used as experimental animals.The unsaturated polypho‐sphoester/β‐tricalcium phosphate composite(UPPE/β‐TCP)and UPPE/β‐TCP composite containing 1% tetracycline(UPPE/β‐TCP/TTC)were injected in circular defects as created in the femoral condyles of rabbits and were left in place for 2 ,4 ,8 ,12 and 24 weeks.The specimens were evaluated morphologically (histology and histomorphometry )and absorption rate of implant at different time points was compared.Single factor analysis of variance and Tukey’s multiple comparison tests were used to deter‐mine statistical significance of results.Results At different implantation stages ,gross examination of retrieved implant/bone composite samples indicated that the UPPE/β‐TCP composite and UPPE/β‐TCP/TTC composite did not evoke inflammatory response ,necrosis or fibrous encapsulation in surrounding bony tissues.Histological examination revealed excellent composite/host bone bonding.At 4 weeks ,resorption induced voids between terminals of bone defects and implants were largely filled with new bone.Composite resorption ,new blood vessels ,osteocytes ,osteons and osteoblast‐like cells lining up with active new bone were observed at remodeling sites.At 12 weeks ,a new bone network was developed within femoral defect ,while composite be‐came islands incorporated in the new bone.At 24 weeks ,bone ingrowth and remodeling activities became so extensive that the interface between residual cement and new bone became less identifiable.In general ,the resorption ratio values increased with implantation time.Conclusion These results showed that UPPE/β‐TCP composite holds promise for use as a synthetic biode‐gradable scaffolds for tissue engineering.