CAJ | 학술논문

采用15个形态学标记和32个CAPS（Cleaved Amplified Polymorphism Sequences，CAPS）标记鉴别73份甜瓜材料。通过15个形态学标记，在遗传相似系数为0.82时将73份甜瓜材料分为3种类型。在分子标记中，被筛选的32对CAPS引物多态性比率为29.63%，PIC值分布在0.22～0.38，平均值为0.34；经过CAPS标记聚类分析，73份甜瓜材料间的遗传相似系数在0.50～0.97之间，当相似系数在0.60时，可以将供试材料分为3种类型。通过构建指纹图谱代码与QR编码，为73份甜瓜材料建立了独特的核酸指纹图谱，每份材料可以相互区别，以便快速对种质资源进行鉴定。
채용15개형태학표기화32개CAPS（Cleaved Amplified Polymorphism Sequences，CAPS）표기감별73빈첨과재료。통과15개형태학표기，재유전상사계수위0.82시장73빈첨과재료분위3충류형。재분자표기중，피사선적32대CAPS인물다태성비솔위29.63%，PIC치분포재0.22～0.38，평균치위0.34；경과CAPS표기취류분석，73빈첨과재료간적유전상사계수재0.50～0.97지간，당상사계수재0.60시，가이장공시재료분위3충류형。통과구건지문도보대마여QR편마，위73빈첨과재료건립료독특적핵산지문도보，매빈재료가이상호구별，이편쾌속대충질자원진행감정。
The 73 melon(Cucumis melo L.)accessions were identified by 15 morphological markers and 32 CAPS markers in this experiment. Using 15 morphological markers,these melons were classified into 3 categories, when the genetic similarity coefficient was 0.82. The polymorphism rate of CAPS markers were 29.63%in molecular markers. The average polymorphism information content(PIC)was 0.34 with a range of 0.22-0.38. The result of CAPS cluster analysis showed that the genetic similarity coefficients of these 73 accessions were ranged from 0.50-0.97. Also,the test materials could be divided into 3 categories,when the genetic similarity coefficient was 0.60. The fingerprinting codes and QR codes had established unique fingerprint for the 73 melon accessions. Each accession could be distinguished from the others. Thus,the germplasm resources could be quickly identified.