CAJ | 학술논문

利用多基因聚合技术，以番茄材料13485（含Cf-5、Tm-1、Mi-1基因）作母本，分别以番茄高代自交系13065（含Ty-2、Ty-3、I-2、Ph-2、Ph-3基因）和高代自交系13337（含耐贮存基因rin）作父本配制杂交组合。利用分子标记辅助选择（MAS）技术对2个组合的F2分离群体的1057个单株进行基因型鉴定，共获得抗5种病害的番茄材料19株，抗4种病害的材料30株，抗3种病害且含rin基因的材料20株。选择田间表现优良的单株留种以进行后续的选择，同时结合人工接种鉴定验证分子标记鉴定的准确性，结果表明，人工接种鉴定与分子标记鉴定结果吻合度均在80%以上。
이용다기인취합기술，이번가재료13485（함Cf-5、Tm-1、Mi-1기인）작모본，분별이번가고대자교계13065（함Ty-2、Ty-3、I-2、Ph-2、Ph-3기인）화고대자교계13337（함내저존기인rin）작부본배제잡교조합。이용분자표기보조선택（MAS）기술대2개조합적F2분리군체적1057개단주진행기인형감정，공획득항5충병해적번가재료19주，항4충병해적재료30주，항3충병해차함rin기인적재료20주。선택전간표현우량적단주류충이진행후속적선택，동시결합인공접충감정험증분자표기감정적준학성，결과표명，인공접충감정여분자표기감정결과문합도균재80%이상。
Taking tomato 13485(containing Cf-5,Tm-1 and Mi-1 gene)as female parent,we crossed it with advanced tomato inbred line 13065(containing Ty-2,Ty-3,I-2,Ph-2 and Ph-3 gene)and advanced tomato inbred line 13337(containing storage tolerant gene rin)as male parents,respectively by pyramiding technology. Then we identified the genotype of 1 057 plants from their F2 segregation populations by molecular marker assistant selection(MAS)technology. We got 19 plants with resistance to 5 diseases,30 plants with resistance to 4 diseases,and 20 plants with resistance to 3 diseases and containing rin gene. We kept those single plants with good field performance as reserve seeds,and will carry out selection afterwards. At the same time,we tested and verified the accuracy of molecular marker by inoculation identification. The result indicated that the goodness of fit by inoculation identification and molecular identification was over 80%.