CAJ | 학술논문

目的：研究模拟失重条件下DNA拓扑异构酶Ⅱβ蛋白表达与分布位置的改变，从而间接了解细胞核基质结构的改变。方法培养大鼠乳鼠颅骨原代成骨细胞并随机分为模拟失重组与对照组。以回转器建立细胞模拟失重模型，之后间接免疫荧光染色与Western blot法检测两组间细胞中DNA拓扑异构酶Ⅱβ的改变。结果与对照组相比，模拟失重组细胞DNA拓扑异构酶Ⅱβ免疫荧光强度降低且失去原有的均匀分布。Western blot检测发现，与对照组相比模拟失重组细胞DNA拓扑异构酶Ⅱβ表达显著降低(P＜0.05)。结论模拟失重可导致成骨细胞DNA拓扑异构酶Ⅱβ表达降低且分布位置不均，提示模拟失重下细胞核基质结构发生改变。
목적：연구모의실중조건하DNA탁복이구매Ⅱβ단백표체여분포위치적개변，종이간접료해세포핵기질결구적개변。방법배양대서유서로골원대성골세포병수궤분위모의실중조여대조조。이회전기건립세포모의실중모형，지후간접면역형광염색여Western blot법검측량조간세포중DNA탁복이구매Ⅱβ적개변。결과여대조조상비，모의실중조세포DNA탁복이구매Ⅱβ면역형광강도강저차실거원유적균균분포。Western blot검측발현，여대조조상비모의실중조세포DNA탁복이구매Ⅱβ표체현저강저(P＜0.05)。결론모의실중가도치성골세포DNA탁복이구매Ⅱβ표체강저차분포위치불균，제시모의실중하세포핵기질결구발생개변。
Objective To investigate the position and expression changes of DNA TopoisomeraseⅡβ, and study the structure changes of nuclear matrix indirectly.Methods Rat calvarial osteoblasts were primarily cultured and randomly divided into simulated microgravity group and control group. After culturing in the condition of simulated microgravity created by clinostat rotation for 48 hours, the DNA TopoisomeraseⅡβ were tested by indirect immunofluorescence and western blot.Results Compared with control group, the DNA TopoisomeraseⅡβfluorescence signal of microgravity group became weak and heterogeneous. Western blot showed that the expression of DNA TopoisomeraseⅡβ in microgravity group were significantly lower than control group (P<0.05).Conclusion Simulated microgravity can decrease the expression and disorganize the distribution of the DNA TopoisomeraseⅡβ, which suggests that the structure of nuclear matrix is changed in simulated microgravity.