CAJ | 학술논문

目的：研究明胶微粒（GP）粒径及明胶与磷酸钙骨水泥（CPC）质量配比这两大因素对GP／CPC复合人工骨材料修复骨缺损的效果。方法：将不同GP粒径（100～200μm、200～300μm）及不同GP与CPC配比（5％GP、10％GP）两两配对后制备GP／CPC复合材料，比较GP／CPC复合材料的孔隙率、抗压强度、表面超微结构、体外生物相容性四大理化特性；构建新西兰兔颅骨缺损模型，在相同质控条件下将上述4组GP／CPC复合人工骨材料进行骨缺损填充，观察并比较其骨修复组织学差异及新骨形成率差异。结果：与粒径100～200μm的GP和5％GP配比组比较，粒径200～300μm的GP和10％GP配比制备的GP／CPC复合材料具有较大的孔径和较高的孔隙率（ P＜0．05），且在兔颅骨缺损修复中有更高的新骨形成量（ P＜0．05）；而100～200μm粒径、5％GP配比组则具有较强的抗压强度（ P＜0．05）。结论：不同GP粒径和含量的GP／CPC复合材料理化特性和修复骨缺损的效果不同，其中GP 200～300μm粒径和10％质量比的GP／CPC复合新型材料更具成骨效果。
목적：연구명효미립（GP）립경급명효여린산개골수니（CPC）질량배비저량대인소대GP／CPC복합인공골재료수복골결손적효과。방법：장불동GP립경（100～200μm、200～300μm）급불동GP여CPC배비（5％GP、10％GP）량량배대후제비GP／CPC복합재료，비교GP／CPC복합재료적공극솔、항압강도、표면초미결구、체외생물상용성사대이화특성；구건신서란토로골결손모형，재상동질공조건하장상술4조GP／CPC복합인공골재료진행골결손전충，관찰병비교기골수복조직학차이급신골형성솔차이。결과：여립경100～200μm적GP화5％GP배비조비교，립경200～300μm적GP화10％GP배비제비적GP／CPC복합재료구유교대적공경화교고적공극솔（ P＜0．05），차재토로골결손수복중유경고적신골형성량（ P＜0．05）；이100～200μm립경、5％GP배비조칙구유교강적항압강도（ P＜0．05）。결론：불동GP립경화함량적GP／CPC복합재료이화특성화수복골결손적효과불동，기중GP 200～300μm립경화10％질량비적GP／CPC복합신형재료경구성골효과。
Objective:To investigate the influence of gelatin particle ( GP) size and gelatin/calcium phosphate cement ( GP/CPC) ratio on repairing potency of comparison artificial bone material .Methods:Composite GP/CPC materials with different GP size (100 ~200 μm vs.200 ~300 μm ) and ratio ( 5% vs.10%) were prepared . Physiochemical and biological properties , including porosity , resistance to compression , ultrastructure and biocompatibility were compared among 4 groups of GP/CPC materials.Different GP/CPC materials were used to repair the critical-size cranial bone defect in rabbit model , and the histology and newly formed bone inside scaffolds ( nBIS) were examined and compared among different groups .Results: GP/CPC with GP of 200 ~300 μm possessed larger micropores than that with GP of 100 ~200μm ( P<0 .05 ) .The GP/CPC containing 10%GP had higher porosity than that containing 5%GP(P<0.05).The animal model study showed that more new bone formed in those defects filled with GP/CPC containing 10% GP with 200 ~300 μm in size compared with GP/CPC containing 5% GP with 100 ~200 μm in size ( P <0.05 ) . While GP/CPC containing 5%GP with 100~200 μm in size showed a higher level of resistance to compression .Conclusion:Both the particle size of GP and its ratio in the GP/CPC affect the properties of the composite biomaterials and their role in bone repairing .In particular , the GP/CPC containing 10%GP with 200~300 μm in size is most suitable for repairing critical-size cranial bone defect in animal model .