现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
16期
2426-2428
,共3页
葛根素%中药制药工艺%工艺学%多元分析%乙醇
葛根素%中藥製藥工藝%工藝學%多元分析%乙醇
갈근소%중약제약공예%공예학%다원분석%을순
Puerarin%Pharmaceutical technology%Technology%Multivariate analysis%Ethanol
目的：优化葛根的提取工艺参数，提高葛根素的提取率。方法在单因素初筛的基础上，以HPLC测定的葛根素含量为响应值，进行三因素三水平Box-Behnken试验设计，并利用Design Expert软件对试验数据进行分析，得到响应面数据并分析结果。结果乙醇浓度与提取时间、提取时间与料液比、乙醇浓度与料液比之间均存在着交互作用。葛根素的最优提取条件为乙醇浓度78%，提取时间为143 min，料液比为1∶10。结论优化的工艺提取条件简便，重现性良好，适用于葛根素的提取。
目的：優化葛根的提取工藝參數，提高葛根素的提取率。方法在單因素初篩的基礎上，以HPLC測定的葛根素含量為響應值，進行三因素三水平Box-Behnken試驗設計，併利用Design Expert軟件對試驗數據進行分析，得到響應麵數據併分析結果。結果乙醇濃度與提取時間、提取時間與料液比、乙醇濃度與料液比之間均存在著交互作用。葛根素的最優提取條件為乙醇濃度78%，提取時間為143 min，料液比為1∶10。結論優化的工藝提取條件簡便，重現性良好，適用于葛根素的提取。
목적：우화갈근적제취공예삼수，제고갈근소적제취솔。방법재단인소초사적기출상，이HPLC측정적갈근소함량위향응치，진행삼인소삼수평Box-Behnken시험설계，병이용Design Expert연건대시험수거진행분석，득도향응면수거병분석결과。결과을순농도여제취시간、제취시간여료액비、을순농도여료액비지간균존재착교호작용。갈근소적최우제취조건위을순농도78%，제취시간위143 min，료액비위1∶10。결론우화적공예제취조건간편，중현성량호，괄용우갈근소적제취。
Objective To optimize the extraction technological parameters of Radix Puerariae in order to improve the ex-traction ratio of puerarin. Methods Based on the single factor preliminary screening ,with the puerarin content detected by HPLC as the response value,the three-factor and three-level Box-Behnken test design was carried on. The experimental data were ana-lyzed by using the Design-Expert software. Results The interaction action existed between the ethanol concentration and extrac-tion time,between the extraction time and solid-liquid ratio and between the ethanol concentration and solid-liquid ratio. The opti-mal extraction conditions of puerarin were the ethanol concentration of 78%,the extraction time of 143 min and the solid to liquid ratio of 1∶10. Conclusion The optimized technological extraction conditions are simple with good reproducibility and suitable for the extraction of puerarin.
