中华妇幼临床医学杂志（电子版）
中華婦幼臨床醫學雜誌（電子版）
중화부유림상의학잡지（전자판）
CHINESE JOURNAL OF OBSTETRICS & GYNECOLOGY AND PEDIATRICS(ELECTRONIC VERSION)
2015年
4期
467-470
,共4页
卵巢囊肿蒂扭转%血清%诊断%手术前期%ROC 曲线
卵巢囊腫蒂扭轉%血清%診斷%手術前期%ROC 麯線
란소낭종체뉴전%혈청%진단%수술전기%ROC 곡선
Cystic ovarian tumors torsion%Serum%Diagnosis%Preoperative period%ROC curve
目的：探讨血清炎性细胞因子白细胞介素(IL)-6、-1β及肿瘤坏死因子(TNF)-α、干扰素-γ在卵巢囊肿蒂扭转患者中的表达及诊断价值。方法选择2011年1月至2014年12月河南省商丘市第一人民医院收治的疑似卵巢囊肿蒂扭转且行手术治疗的40例患者为研究对象,按照术中探查显示是否为卵巢囊肿蒂扭转,将其分为研究组(n=23,术中确诊为卵巢囊肿蒂扭转患者)和对照组(n=17,术中确诊为非卵巢囊肿蒂扭转患者)。采用酶联免疫吸附测定(ELISA)法检测40例 患 者 肘 静 脉 血 血 清 炎 性 细 胞 因 子IL-6、-1β及 TNF-α、干扰素-γ表达水平,并对两组患者血清炎性细胞因子(IL-6、-1β及 TNF-α、干扰素-γ)的检测结果进行统计学分析,并绘制受试者操作特征(ROC)曲线,计算各血清炎性细胞因子在诊断卵巢囊肿蒂扭转的临界值、敏感度、特异度、阳性预测值、阴性预测值及约登指数。本研究遵循的程序符合河南省商丘市第一人民医院人体试验委员会制定的伦理学标准,得到该委员会批准,研究征得受试对象的知情同意,并与之签订临床研究知情同意书。结果研究组患者 IL-6、-1β及 TNF-α、干扰素-γ表达水平均高于对照组[IL-6：(10.92±0.63)pg/mL vs (10.20±0.58)pg/mL；IL-1β：(53.28±15.29)pg/mL vs (34.59±10.27)pg/mL；TNF-α：(8.96±1.50)pg/mL vs (8.02±1.34)pg/mL；干扰素-γ：(25.17 ±6.56)pg/mL vs (16.52±3.71)pg/mL],并且差异均有统计学意义(t =3.69,4.36,2.05,4.88；P =0.0001,0.0001,0.0053,0.034)。通过绘制 ROC 曲线得出,IL-6与 IL-1β曲线下面积分别为0.838与0.831,对卵巢囊肿蒂扭转诊断的敏感度分别为78.3%和91.3%,特异度分别为82.5%%和82.4%。结论血清 IL-6与IL-1β可作为卵巢囊肿蒂扭转的术前辅助诊断指标。
目的：探討血清炎性細胞因子白細胞介素(IL)-6、-1β及腫瘤壞死因子(TNF)-α、榦擾素-γ在卵巢囊腫蒂扭轉患者中的錶達及診斷價值。方法選擇2011年1月至2014年12月河南省商丘市第一人民醫院收治的疑似卵巢囊腫蒂扭轉且行手術治療的40例患者為研究對象,按照術中探查顯示是否為卵巢囊腫蒂扭轉,將其分為研究組(n=23,術中確診為卵巢囊腫蒂扭轉患者)和對照組(n=17,術中確診為非卵巢囊腫蒂扭轉患者)。採用酶聯免疫吸附測定(ELISA)法檢測40例 患 者 肘 靜 脈 血 血 清 炎 性 細 胞 因 子IL-6、-1β及 TNF-α、榦擾素-γ錶達水平,併對兩組患者血清炎性細胞因子(IL-6、-1β及 TNF-α、榦擾素-γ)的檢測結果進行統計學分析,併繪製受試者操作特徵(ROC)麯線,計算各血清炎性細胞因子在診斷卵巢囊腫蒂扭轉的臨界值、敏感度、特異度、暘性預測值、陰性預測值及約登指數。本研究遵循的程序符閤河南省商丘市第一人民醫院人體試驗委員會製定的倫理學標準,得到該委員會批準,研究徵得受試對象的知情同意,併與之籤訂臨床研究知情同意書。結果研究組患者 IL-6、-1β及 TNF-α、榦擾素-γ錶達水平均高于對照組[IL-6：(10.92±0.63)pg/mL vs (10.20±0.58)pg/mL；IL-1β：(53.28±15.29)pg/mL vs (34.59±10.27)pg/mL；TNF-α：(8.96±1.50)pg/mL vs (8.02±1.34)pg/mL；榦擾素-γ：(25.17 ±6.56)pg/mL vs (16.52±3.71)pg/mL],併且差異均有統計學意義(t =3.69,4.36,2.05,4.88；P =0.0001,0.0001,0.0053,0.034)。通過繪製 ROC 麯線得齣,IL-6與 IL-1β麯線下麵積分彆為0.838與0.831,對卵巢囊腫蒂扭轉診斷的敏感度分彆為78.3%和91.3%,特異度分彆為82.5%%和82.4%。結論血清 IL-6與IL-1β可作為卵巢囊腫蒂扭轉的術前輔助診斷指標。
목적：탐토혈청염성세포인자백세포개소(IL)-6、-1β급종류배사인자(TNF)-α、간우소-γ재란소낭종체뉴전환자중적표체급진단개치。방법선택2011년1월지2014년12월하남성상구시제일인민의원수치적의사란소낭종체뉴전차행수술치료적40례환자위연구대상,안조술중탐사현시시부위란소낭종체뉴전,장기분위연구조(n=23,술중학진위란소낭종체뉴전환자)화대조조(n=17,술중학진위비란소낭종체뉴전환자)。채용매련면역흡부측정(ELISA)법검측40례 환 자 주 정 맥 혈 혈 청 염 성 세 포 인 자IL-6、-1β급 TNF-α、간우소-γ표체수평,병대량조환자혈청염성세포인자(IL-6、-1β급 TNF-α、간우소-γ)적검측결과진행통계학분석,병회제수시자조작특정(ROC)곡선,계산각혈청염성세포인자재진단란소낭종체뉴전적림계치、민감도、특이도、양성예측치、음성예측치급약등지수。본연구준순적정서부합하남성상구시제일인민의원인체시험위원회제정적윤리학표준,득도해위원회비준,연구정득수시대상적지정동의,병여지첨정림상연구지정동의서。결과연구조환자 IL-6、-1β급 TNF-α、간우소-γ표체수평균고우대조조[IL-6：(10.92±0.63)pg/mL vs (10.20±0.58)pg/mL；IL-1β：(53.28±15.29)pg/mL vs (34.59±10.27)pg/mL；TNF-α：(8.96±1.50)pg/mL vs (8.02±1.34)pg/mL；간우소-γ：(25.17 ±6.56)pg/mL vs (16.52±3.71)pg/mL],병차차이균유통계학의의(t =3.69,4.36,2.05,4.88；P =0.0001,0.0001,0.0053,0.034)。통과회제 ROC 곡선득출,IL-6여 IL-1β곡선하면적분별위0.838여0.831,대란소낭종체뉴전진단적민감도분별위78.3%화91.3%,특이도분별위82.5%%화82.4%。결론혈청 IL-6여IL-1β가작위란소낭종체뉴전적술전보조진단지표。
Objective To investigate a possible role for serum inflammatory cytokines [interleukin (IL)-6,1β and tumor necrosis factor (TNF)-α,interferon-γ]as pre-operative diagnosis index of ovarian cyst torsion.Methods From January 201 1 to December 2014,a total of forty cases who were admitted to Shangqiu First People′s Hospital with suspected clinical diagnosis of ovarian cyst torsion were prospectively assigned to the study.They were divided into two groups according to surgery examination,study group (n=23,ovarian cyst torsion patients)and control group (n=1 7,non-ovarian cyst torsion patients).Blood samples were drawn pre-operatively and examined for serum levels of IL-6,-1βand TNF-α,interferon-γby enzyme-linked immunosorbent assay (ELISA ). The levels of serum inflammatory cytokines, receiver operating characteristic (ROC)curve between two groups were analyzed.Meanwhile,the critical value, sensitivity,specificity,positive predictive value,negative predictive value and Youden′s index of serum inflammatory cytokines in the diagnosis of ovarian cyst torsion were detected.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Shangqiu First People′s Hospital in Henan Province.Informed consent was obtained from each participates.Results Levels of IL-6,-1βand TNF-α,interferon-γin study group were higher than those in group control[IL-6:(10.92±0.63)pg/mL vs (10.20±0.58)pg/mL;IL- 1 β :(53.28±1 5.29)pg/mL vs (34.59±10.27)pg/mL;TNF-α:(8.96± 1.50)pg/mL vs (8.02±1.34)pg/mL;interferon-γ:(25.1 7 ±6.56)pg/mL vs (1 6.52 ±3.71)pg/mL], and all the differences were statistically significant (t = 3.69,4.36,2.05,4.88;P = 0.000 1,0.000 1, 0.005 3,0.034).According to the ROC curve,the area under curve of IL-6,-1βwere 0.838 and 0.831,the sensitivity of diagnosing ovarian cyst torsion were 78.3% and 91.3%,the specificity were 82.5% and 82.4%, respectively.Conclusions Elevated serum levels of IL-6 and IL-1β were associated with the occurrence of ovarian cyst torsion.In combination with vague clinical signs of ovarian cyst torsion,serum IL-6 and IL-1βcould be the pre-operative diagnostic indices of ovarian cyst torsion.
