天津科技
天津科技
천진과기
TIANJIN SCIENCE & TECHNOLOGY
2015年
8期
19-21
,共3页
刘达综%高慧珊%刘娜%何洁明%徐树兰%郑常格%吕思行
劉達綜%高慧珊%劉娜%何潔明%徐樹蘭%鄭常格%呂思行
류체종%고혜산%류나%하길명%서수란%정상격%려사행
白僵菌%分离%鉴定
白僵菌%分離%鑒定
백강균%분리%감정
Beauveria bassiana%isolation%identification
利用PDA培养基，对收集自广东茂名的玉米螟(Pyrausta nubilalis)病原真菌(编号：Bba1菌株)进行分离培养。对 Bba1菌株的菌落形态、产孢结构和分生孢子形态、大小进行观察和测量，从形态学初步鉴定该菌株为球孢白僵菌(Beauveria bassiana)；同时对Bba1菌株的26,s rDNA和rDNA ITS区域进行序列分析，与NCBI数据库中球孢白僵菌的同源性达到99%,以上。最终判定来自玉米螟的病原真菌Bba1菌株为球孢白僵菌(Beauveria bassiana)。
利用PDA培養基，對收集自廣東茂名的玉米螟(Pyrausta nubilalis)病原真菌(編號：Bba1菌株)進行分離培養。對 Bba1菌株的菌落形態、產孢結構和分生孢子形態、大小進行觀察和測量，從形態學初步鑒定該菌株為毬孢白僵菌(Beauveria bassiana)；同時對Bba1菌株的26,s rDNA和rDNA ITS區域進行序列分析，與NCBI數據庫中毬孢白僵菌的同源性達到99%,以上。最終判定來自玉米螟的病原真菌Bba1菌株為毬孢白僵菌(Beauveria bassiana)。
이용PDA배양기，대수집자엄동무명적옥미명(Pyrausta nubilalis)병원진균(편호：Bba1균주)진행분리배양。대 Bba1균주적균락형태、산포결구화분생포자형태、대소진행관찰화측량，종형태학초보감정해균주위구포백강균(Beauveria bassiana)；동시대Bba1균주적26,s rDNA화rDNA ITS구역진행서렬분석，여NCBI수거고중구포백강균적동원성체도99%,이상。최종판정래자옥미명적병원진균Bba1균주위구포백강균(Beauveria bassiana)。
A strain(No.Bba1)was isolated using PDA culture medium from the muscardine cadaver ofPyrausta nubilalis collected from Maoming in Guangdong Province.In this study,the colony morphology,spore structure,spore shape and size of Bba1 strain were observed and measured,and it was initially identified asBeauveria bassiana.At the same time,its 26,s rDNA and rDNA ITS were sequenced and then compared with those ofBeauveria bassiana in NCBI database.The re-sults showed the homology was above 99%,.Eventually,Bba1 strain was identified asBeauveria bassiana.
