CAJ | 학술논문

目的：初步研究 IL-25通过 IL-4和IL-13促进哮喘小鼠早期气道重塑，探讨 IL-25在早期哮喘气道重塑中的作用。方法将40只BALB/C雌性小鼠随机均分为哮喘组、对照组、IL-25组和抗IL-25组，哮喘组、IL-25组和抗IL-25组是用鸡卵白蛋白（OVA）激发和致敏建立哮喘模型，其中IL-25组、抗IL-25组分别于每次致敏前1 h鼻腔内滴入重组小鼠IL-25、抗重组小鼠IL-25，对照组是用0.9%生理盐水替代。用酶联免疫吸附试验（ELISA）测定血清和肺泡灌洗液中IL-4和IL-13的表达，用 HE 染色观察各组气道基底膜厚度，用免疫组织化学法测定气道中α-平滑肌肌动蛋白（α-SMA）的表达量（气道基底膜厚度和α-SMA均为气道重塑的重要指标）。结果 ELISA显示：与哮喘组相比，在血清和肺泡灌洗液中IL-25组的IL-4和IL-13表达量明显增加（P＜0.05），抗IL-25组中IL-4和IL-13表达量明显降低（P＜0.05）；HE染色显示：与哮喘组相比，IL-25组支气管管壁增厚明显、管腔狭窄严重，气道炎性细胞浸润明显增加，黏膜上皮破损严重，抗IL-25组管壁厚度、管腔狭窄、炎症细胞浸润及黏膜上皮破损较哮喘组明显减轻；免疫组织化学的结果显示：与哮喘组相比，IL-25组支气管壁α-SMA蛋白表达明显增加（P＜0.05），而抗IL-25组α-SMA蛋白表达则明显减少（P＜0.05）。结论 IL-25通过IL-4和IL-13促进气道上皮细胞的增生，炎性细胞的聚集、浸润，黏液分泌物的增加，可能对哮喘小鼠早期气道重塑起到一定的促进作用。
목적：초보연구 IL-25통과 IL-4화IL-13촉진효천소서조기기도중소，탐토 IL-25재조기효천기도중소중적작용。방법장40지BALB/C자성소서수궤균분위효천조、대조조、IL-25조화항IL-25조，효천조、IL-25조화항IL-25조시용계란백단백（OVA）격발화치민건립효천모형，기중IL-25조、항IL-25조분별우매차치민전1 h비강내적입중조소서IL-25、항중조소서IL-25，대조조시용0.9%생리염수체대。용매련면역흡부시험（ELISA）측정혈청화폐포관세액중IL-4화IL-13적표체，용 HE 염색관찰각조기도기저막후도，용면역조직화학법측정기도중α-평활기기동단백（α-SMA）적표체량（기도기저막후도화α-SMA균위기도중소적중요지표）。결과 ELISA현시：여효천조상비，재혈청화폐포관세액중IL-25조적IL-4화IL-13표체량명현증가（P＜0.05），항IL-25조중IL-4화IL-13표체량명현강저（P＜0.05）；HE염색현시：여효천조상비，IL-25조지기관관벽증후명현、관강협착엄중，기도염성세포침윤명현증가，점막상피파손엄중，항IL-25조관벽후도、관강협착、염증세포침윤급점막상피파손교효천조명현감경；면역조직화학적결과현시：여효천조상비，IL-25조지기관벽α-SMA단백표체명현증가（P＜0.05），이항IL-25조α-SMA단백표체칙명현감소（P＜0.05）。결론 IL-25통과IL-4화IL-13촉진기도상피세포적증생，염성세포적취집、침윤，점액분비물적증가，가능대효천소서조기기도중소기도일정적촉진작용。
ObjectiveTo explore the role of IL-25 in early airway remodeling of asthma on the regulation of IL-4 and IL-13.MethodsSerum and BALF were colected from forty BALB/C female mice which were randomly divided into asthma group, control group, IL-25 group and anti IL-25 group. Asthma model was established by inhaling chicken ovalbumin (OVA) in asthma group, IL-25 group and anti IL-25 group. One hour before every sensitization, IL-25 restructuring mice and anti IL-25 restructuring mice were respectively dripped into the noses of the IL-25 group and anti IL-25 group, while the control group with 0.9% saline instead. The level of IL-4 and IL-13 in serum and BALF were determined by ELISA. Basement membrane thickness as an important index of airway remolding was detected by HE staining. The expressing ofα-SMA was determined by immnohistochemistry.ResultsELISA showed that compared with the asthma group, the expression of IL-4 and IL-13 of the serum and BALF of IL-25 group increased significantly (P<0.05), while the expression in anti IL-25 groups decreased obviously (P<0.05).The HE staining showed that compared with the asthma group, the thickness of bronchial wal and the stenosis of bronchial lumen and the airway inflammatory cels infiltration and the damage of mucosal epithelium of IL-25 group increased obviously. While the thickness of bronchial wal and the stenosis of bronchial lumen and the airway inflammatory cels infiltration and the damage of mucosal epithelium of anti IL-25 group decreased significantly. The immunohistochemical showed that compared with the asthma group, the expression of α-SMA of bronchial wal of IL-25 group increased significantly (P<0.05), while the expression in anti IL-25 groups decreased obviously (P<0.05).ConclusionIL-25 has the potential to promote airway remoding through IL-4 and IL-13, which can promote the hyperplasia of the airway epithelial cels, the accumulation of inflammatory cels, infiltration, mucus secretion increased.