CAJ | 학술논문

采用高效液相色谱-四极杆／飞行时间质谱对中药保健品中红地那非、伐地那非、那红地那非、他达拉非、西地那非、豪莫西地那非、硫代艾地那非、伪伐地那非、羟基豪莫西地那非、育亨宾、达泊西汀等11种非法添加的壮阳类药物进行了快速筛查、确证和定性定量检测。样品经甲醇提取后，采用正离子扫描模式进行检测。结果表明，11种待测物的定量限除那红地那非为25.0 ng／mL外，其他10种壮阳类药物均为5.0 ng／mL，在5.0~200.0 ng／mL（那红地那非在25.0~500.0 ng／mL）范围内，线性相关性较好，相关系数均不小于0.9990。平均添加回收率为82.0％~105.9％，相对标准偏差为4.7％~16.5％。本方法利用准确质量数匹配和自建标准谱库，通过多级特征碎片离子的比对，实现快速筛查和确证，简单快速，结果可靠。
채용고효액상색보-사겁간／비행시간질보대중약보건품중홍지나비、벌지나비、나홍지나비、타체랍비、서지나비、호막서지나비、류대애지나비、위벌지나비、간기호막서지나비、육형빈、체박서정등11충비법첨가적장양류약물진행료쾌속사사、학증화정성정량검측。양품경갑순제취후，채용정리자소묘모식진행검측。결과표명，11충대측물적정량한제나홍지나비위25.0 ng／mL외，기타10충장양류약물균위5.0 ng／mL，재5.0~200.0 ng／mL（나홍지나비재25.0~500.0 ng／mL）범위내，선성상관성교호，상관계수균불소우0.9990。평균첨가회수솔위82.0％~105.9％，상대표준편차위4.7％~16.5％。본방법이용준학질량수필배화자건표준보고，통과다급특정쇄편리자적비대，실현쾌속사사화학증，간단쾌속，결과가고。
A method was established for rapid screening and quantifying 11 illegally added anti-impotence preparations( yohimbine,acetildenafil,nor-acetildenafil,homosildenafil,hydroxy-homosildenafil,sildenafil,vardenafil,thioaildenafil,tadalafil,pseudovardenafil,dapoxetine ) in health care products by high performance liquid chromatography-high resolution mass spec-trometry. The samples were extracted with methanol and analyzed by positive mode in the MS detection. The results showed that the limits of detection were 25. 0 ng/mLexcept for nor-acetil-denafil( 5. 0 ng/mL ),the linear ranges were 5. 0-200. 0 ng/mL except for nor-acetildenafil (25. 0-500. 0 ng/mL)with the correlation coefficients not less than 0. 999 0. The recoveries were in the range of 82. 0%-105. 9% with the relative standard deviations of 4. 7%-16. 5%. This method is accurate,simple and rapid,and can be used in rapid screening and quantitative anal-ysis of the 11 illegally added anti-impotence in health care products.