CAJ | 학술논문

目的：初步研究尿hepcidin-25水平在儿童铁缺乏症（ IDS）早期诊断中的诊断性能。方法采用酶联免疫吸附试验（ ELISA）检测25例贮存铁耗尽（ ID）阶段患儿、25例缺铁性红细胞生成（ IDE）阶段患儿、25例缺铁性贫血（ IDA）患儿和25名健康儿童（正常对照组）尿hepcidin-25浓度。采用受试者工作特征（ ROC）曲线确定尿hepcidin-25诊断ID、IDE、IDA的临界值及其相应的诊断性能。结果 ID组、IDE组、IDA组及正常对照组两两之间尿hepcidin-25水平差异均有统计学意义（ P均＜0．05）。尿hepcidin-25对鉴别正常对照组与ID组、IDE组、IDA组的ROC曲线下面积分别为0．865、0．974、0．998；鉴别IDE组与IDA组、ID组、正常对照和ID混合组的ROC曲线下面积分别为0．872、0．870、0．922。结论尿hepcidin-25检测是一项简单而没有创伤的试验，在儿童IDS的早期诊断中具有一定的应用价值。
목적：초보연구뇨hepcidin-25수평재인동철결핍증（ IDS）조기진단중적진단성능。방법채용매련면역흡부시험（ ELISA）검측25례저존철모진（ ID）계단환인、25례결철성홍세포생성（ IDE）계단환인、25례결철성빈혈（ IDA）환인화25명건강인동（정상대조조）뇨hepcidin-25농도。채용수시자공작특정（ ROC）곡선학정뇨hepcidin-25진단ID、IDE、IDA적림계치급기상응적진단성능。결과 ID조、IDE조、IDA조급정상대조조량량지간뇨hepcidin-25수평차이균유통계학의의（ P균＜0．05）。뇨hepcidin-25대감별정상대조조여ID조、IDE조、IDA조적ROC곡선하면적분별위0．865、0．974、0．998；감별IDE조여IDA조、ID조、정상대조화ID혼합조적ROC곡선하면적분별위0．872、0．870、0．922。결론뇨hepcidin-25검측시일항간단이몰유창상적시험，재인동IDS적조기진단중구유일정적응용개치。
Objective To study the diagnostic performance of urinary hepcidin-25 for the early-stage diagnosis of iron deficient syndrome ( IDS) among children.Methods By enzyme-linked immunosorbent assay ( ELISA) , urinary hepcidin-25 levels were determined in 25 cases of iron depletion( ID) stage, 25 cases of iron deficient erythropoiesis ( IDE) stage, 25 cases of iron deficient anemia( IDA) and 25 healthy children ( control group) .The cut-off values of urinary hepcidin-25 for ID, IDE and IDA and corresponding diagnostic performance were analyzed by receiver operating characteristic( ROC) curve.Results There was statistical significance of urinary hepcidin-25 levels in comparison between any 2 groups of ID, IDE, IDA and control groups ( P <0.05 ) .The areas under ROC curves for urinary hepcidin-25 for differentiating ID, IDE and IDA groups from control group were 0.865, 0.974 and 0.998, respectively. The areas under ROC curves for urinary hepcidin-25 for differentiating IDE from IDA, differentiating IDE from ID, differentiating IDE from the mixing group of control with ID were 0.872, 0.870 and 0.922, respectively.Conclusions The determination of urinary hepcidin-25 level is a simple and non-invasive test, and it might have application significance in the early-stage diagnosis of IDS among children.