声学技术
聲學技術
성학기술
Technical Acousitics
2015年
4期
333-337
,共5页
低频低能量超声%微泡造影剂%前列腺癌%原子力声显微镜%细胞自噬
低頻低能量超聲%微泡造影劑%前列腺癌%原子力聲顯微鏡%細胞自噬
저빈저능량초성%미포조영제%전렬선암%원자력성현미경%세포자서
low-frequency ultrasound%microbubble contrast agent%prostate cancer%Atomic Force Acoustic Micro-scope(AFAM)%autophage
目的：采用原子力声显微镜及透射电镜观察低频低能量超声联合微泡对前列腺癌细胞 DU145及正常前列腺上皮细胞RWPE-1的作用。方法：两种细胞均分为对照组、单纯超声组、超声联合微泡组。对照组加入一定比例的生理盐水，不进行超声辐照；单纯超声组加入相同比例的生理盐水，用发射频率为21 kHz的低频超声辐照，辐照2 min，占空比30%；超声联合微泡组加入同前相同比例的微泡造影剂悬浊液，用与单纯超声组相同的超声辐照。处理过的细胞立即用原子力声显微镜观察其形貌并计算其杨氏模量。同时，对同一处理方式的对照组及超声联合微泡组细胞继续培养24 h后，用透射电镜观察细胞。结果：单纯超声组的DU145细胞及RWPE-1细胞的细胞形态与对照组无明显变化；超声联合微泡组的DU145细胞及RWPE-1细胞形态呈类圆形，细胞表面可见放射状显微丝状结构，细胞膜表面可见多个大孔状结构；超声联合微泡组的DU145细胞的弹性模量较RWPE-1细胞大，且与对照组相比，两种细胞的弹性模量均变大， DU145细胞尤甚。透射电镜观察结果示对照组的两种细胞未见细胞自噬，而超声联合微泡组两种细胞都出现自噬现象，其中 DU145细胞中可见凋亡现象。结论：低频低能量超声联合微泡可引起前列腺癌 DU145细胞及正常前列腺上皮RWPE-1细胞的细胞膜出现孔状结构，并诱导其自噬，且对DU145细胞的损伤大于RWPE-1细胞。
目的：採用原子力聲顯微鏡及透射電鏡觀察低頻低能量超聲聯閤微泡對前列腺癌細胞 DU145及正常前列腺上皮細胞RWPE-1的作用。方法：兩種細胞均分為對照組、單純超聲組、超聲聯閤微泡組。對照組加入一定比例的生理鹽水，不進行超聲輻照；單純超聲組加入相同比例的生理鹽水，用髮射頻率為21 kHz的低頻超聲輻照，輻照2 min，佔空比30%；超聲聯閤微泡組加入同前相同比例的微泡造影劑懸濁液，用與單純超聲組相同的超聲輻照。處理過的細胞立即用原子力聲顯微鏡觀察其形貌併計算其楊氏模量。同時，對同一處理方式的對照組及超聲聯閤微泡組細胞繼續培養24 h後，用透射電鏡觀察細胞。結果：單純超聲組的DU145細胞及RWPE-1細胞的細胞形態與對照組無明顯變化；超聲聯閤微泡組的DU145細胞及RWPE-1細胞形態呈類圓形，細胞錶麵可見放射狀顯微絲狀結構，細胞膜錶麵可見多箇大孔狀結構；超聲聯閤微泡組的DU145細胞的彈性模量較RWPE-1細胞大，且與對照組相比，兩種細胞的彈性模量均變大， DU145細胞尤甚。透射電鏡觀察結果示對照組的兩種細胞未見細胞自噬，而超聲聯閤微泡組兩種細胞都齣現自噬現象，其中 DU145細胞中可見凋亡現象。結論：低頻低能量超聲聯閤微泡可引起前列腺癌 DU145細胞及正常前列腺上皮RWPE-1細胞的細胞膜齣現孔狀結構，併誘導其自噬，且對DU145細胞的損傷大于RWPE-1細胞。
목적：채용원자력성현미경급투사전경관찰저빈저능량초성연합미포대전렬선암세포 DU145급정상전렬선상피세포RWPE-1적작용。방법：량충세포균분위대조조、단순초성조、초성연합미포조。대조조가입일정비례적생리염수，불진행초성복조；단순초성조가입상동비례적생리염수，용발사빈솔위21 kHz적저빈초성복조，복조2 min，점공비30%；초성연합미포조가입동전상동비례적미포조영제현탁액，용여단순초성조상동적초성복조。처리과적세포립즉용원자력성현미경관찰기형모병계산기양씨모량。동시，대동일처리방식적대조조급초성연합미포조세포계속배양24 h후，용투사전경관찰세포。결과：단순초성조적DU145세포급RWPE-1세포적세포형태여대조조무명현변화；초성연합미포조적DU145세포급RWPE-1세포형태정류원형，세포표면가견방사상현미사상결구，세포막표면가견다개대공상결구；초성연합미포조적DU145세포적탄성모량교RWPE-1세포대，차여대조조상비，량충세포적탄성모량균변대， DU145세포우심。투사전경관찰결과시대조조적량충세포미견세포자서，이초성연합미포조량충세포도출현자서현상，기중 DU145세포중가견조망현상。결론：저빈저능량초성연합미포가인기전렬선암 DU145세포급정상전렬선상피RWPE-1세포적세포막출현공상결구，병유도기자서，차대DU145세포적손상대우RWPE-1세포。
Objective: To explore the impact of 21 kHz low-intensity ultrasound combined with microbubbles on both DU145 cells and RWPE-1 cells by using Atomic force acoustic microscope (AFAM) and Transmission electron microscopy (TEM).Methods: Both DU145 cells and RWPE-1 cells were divided into three groups: control group, ultrasound group and ultrasound combined with microbubbles group. Conditions of the low-frequency and low-energy ultrasound (US) irradia-tion were: Frequency, 21 kHz; Exposure time, 2 min at a duty ratio of 30%; and the valid treatment time of 84 seconds was used for the combination with microbubbles (100μl/ml). Cells of different groups were imaged in cover slips by AFAM immediately after the intervention. Twenty-four hours after intervention, TEM was used to observe cells.Results: Cellular membrane and three-dimensional structure of both cells showed significant difference in ultrasound combined with mi-crobubbles group compared with control group, while no difference in ultrasound group compared with control group. Compared with control group, the elasticity modulus of both cells in ultrasound combined with microbubbles group was higher, and also the elasticity modulus of DU145 cells was higher than RWPE-1 in ultrasound combined with microbubbles group. Lots of autophagosomes or autolysosomes were detected by TEM in both cells in ultrasound combined with mi-crobubbles group. TEM also demonstrated some apoptosis of DU145 cells in ultrasound combined with microbubbles group.Conclusions: The low-frequency ultrasound combined with microbubbles can induce autophagy in both DU145 cells and RWPE-1 cells, and the injury to DU145 cells is more serious than to RWPE-1 cells.
