CAJ | 학술논문

采用高效液相色谱-蒸发光散射检测器（ HPLC-ELSD）来研究不同产地龙葵药材品质的差异，为龙葵药材质量标准的制定提供参考依据。使用 Phenomenex C18色谱柱，流动相为乙腈-20％（ v／v）甲醇水溶液（含0.03％（ v／v）三乙胺），梯度洗脱，蒸发管温度为40℃。利用“中药色谱指纹图谱相似度评价系统”对所采集的色谱图进行相似度比对，选择相似度在0.9~1.0之间的色谱图归一化拟合成龙葵的标准指纹图谱，并利用对照品进行色谱峰指认。研究结果表明，不同产地来源的龙葵药材之间具有显著的差异，依据 HPLC-ELSD采集到的非挥发类成分可对中药材进行区分。该方法为龙葵药材的真实性、优良性和稳定性评价提供了一种新的、具有广阔应用前景的技术手段。
채용고효액상색보-증발광산사검측기（ HPLC-ELSD）래연구불동산지룡규약재품질적차이，위룡규약재질량표준적제정제공삼고의거。사용 Phenomenex C18색보주，류동상위을정-20％（ v／v）갑순수용액（함0.03％（ v／v）삼을알），제도세탈，증발관온도위40℃。이용“중약색보지문도보상사도평개계통”대소채집적색보도진행상사도비대，선택상사도재0.9~1.0지간적색보도귀일화의합성룡규적표준지문도보，병이용대조품진행색보봉지인。연구결과표명，불동산지래원적룡규약재지간구유현저적차이，의거 HPLC-ELSD채집도적비휘발류성분가대중약재진행구분。해방법위룡규약재적진실성、우량성화은정성평개제공료일충신적、구유엄활응용전경적기술수단。
Solanum nigrum L. contains a variety of active ingredients. It has been applied broadly in clinical practice. However,the compositions of Solanum nigrum L. from different cultivated areas are quite different. Therefore,it is necessary to set up a standard fingerprint. In this work,a series of Solanum nigrum L. samples were collected from different habitats. A high performance liquid chromatography with evaporative light-scattering detection ( HPLC-ELSD)method was developed to study the differences in quality of these samples. The method was optimized by comparing different columns,gradient or isocratic elution and temperatures of evaporating tube. A Phenomenex C18 column was finally selected for separation with gradient elution. The mobile phases were acetonitrile-20%( v/v)methanol aqueous solution containing 0. 03%( v/v)triethylamine. The temperature of evaporating tube was set at 40 ℃. The valida-tion was performed and the results showed that the method had good precision,repeatability and stability. The validated method was applied to detect Solanum nigrum L. from different habitats. The similarities of the acquired chromatograms were analyzed by using the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( TCM ). The chromatograms with a similarity between 0. 9 and 1. 0 were normalized and fitted to form a standard fingerprint of Solanum nigrum L. Thirteen common peaks were found and eleven peaks were identified with authentic standards. The results showed that remarkable differences were found among Solanum nigrum L. from different habitats. These herbs could be distin-guished based on the compositions detected by HPLC-ELSD. This method provided a technology to evaluate the homogeneity and stability of Solanum nigrum L with wide application prospects and practical value.