CAJ | 학술논문

采用硫酸除钙和调节 pH的样品预处理方法，利用高效液相色谱对菌群降解纤维素发酵液中的糖、有机酸和醇3类物质进行分析，待测组分与培养基成分能够得到有效分离。从菌群降解纤维素发酵液中检出纤维二糖、葡萄糖、乙醇、丁醇、甘油、乙酸与丁酸并进行了定量分析，7种组分的检出限范围为0.10~2.00 mg／L，线性相关系数均大于0.9996，线性范围为0.020~1.000 g／L，回收率为85.41％~115.60％，相对标准偏差（ RSD ）为0.22％~4.62％（ n=6）。该方法准确可靠，可实现对菌群降解纤维素发酵液中糖、醇和有机酸的同时准确测定。
채용류산제개화조절 pH적양품예처리방법，이용고효액상색보대균군강해섬유소발효액중적당、유궤산화순3류물질진행분석，대측조분여배양기성분능구득도유효분리。종균군강해섬유소발효액중검출섬유이당、포도당、을순、정순、감유、을산여정산병진행료정량분석，7충조분적검출한범위위0.10~2.00 mg／L，선성상관계수균대우0.9996，선성범위위0.020~1.000 g／L，회수솔위85.41％~115.60％，상대표준편차（ RSD ）위0.22％~4.62％（ n=6）。해방법준학가고，가실현대균군강해섬유소발효액중당、순화유궤산적동시준학측정。
A high performance liquid chromatographic method was established for the determi-nation of metabolites( sugars,organic acids and alcohols)in microbial consortium fermenta-tion broth from cellulose. Sulfate was first added in the samples to precipitate calcium ions in microbial consortium culture medium and lower the pH of the solution to avoid the dissociation of organic acids,then the filtrates were effectively separated using high performance liquid chromatography. Cellobiose,glucose,ethanol,butanol,glycerol,acetic acid and butyric acid were quantitatively analyzed. The detection limits were in the range of 0. 10-2. 00 mg/L. The linear correlation coefficients were greater than 0. 999 6 in the range of 0. 020 to 1.000 g/L. The recoveries were in the range of 85. 41%-115. 60% with the relative standard deviations of 0. 22%-4. 62%(n=6). This method is accurate for the quantitative analysis of the alcohols,organic acids and saccharides in microbial consortium fermentation broth from cellulose.