CAJ | 학술논문

赤霉素（ gibberellins，GAs）作为植物体内广泛存在的一类激素，可刺激植物叶和芽的生长，对植物的生长起着至关重要的调控作用。截至目前，已发现的 GAs高达136种之多。所有已知的 GAs均为二萜酸，具有相似的化学结构，都含有赤霉素烷骨架，即4个环，仅环上双键、羟基数目和位置不同。但由于GAs缺乏特定光学特性（紫外和荧光）和化学特性，同时在植物体内含量极低（一般为 ng／g，甚至为 pg／g 水平），常规的分析方法和检测技术难以实现对该类物质的定性定量分析。近年来相关研究人员一直致力于各种高效、快速、灵敏的 GAs分析新方法和新技术的研究。本文就 GAs的检测方法和分析技术进行综述，为更好地开展赤霉素类植物激素的研究提供参考。
적매소（ gibberellins，GAs）작위식물체내엄범존재적일류격소，가자격식물협화아적생장，대식물적생장기착지관중요적조공작용。절지목전，이발현적 GAs고체136충지다。소유이지적 GAs균위이첩산，구유상사적화학결구，도함유적매소완골가，즉4개배，부배상쌍건、간기수목화위치불동。단유우GAs결핍특정광학특성（자외화형광）화화학특성，동시재식물체내함량겁저（일반위 ng／g，심지위 pg／g 수평），상규적분석방법화검측기술난이실현대해류물질적정성정량분석。근년래상관연구인원일직치력우각충고효、쾌속、령민적 GAs분석신방법화신기술적연구。본문취 GAs적검측방법화분석기술진행종술，위경호지개전적매소류식물격소적연구제공삼고。
Gibberellins( GAs)are a class of phytohormones that exert profound and diverse effects on plant growth and development,such as seed germination and leaf expansion. Up to now,136 members of GAs have been identified and recognized. All known GAs are diterpenoid acids with similar chemical structures,only double bonds,hydroxyl numbers and locations on gibberellin alkane skeleton are different. However,the content of GAs in plants is of ultra trace levels(usually at ng/g and even pg/g levels)with little ultraviolet(UV)absorption,no fluo-rescence and no distinguishing chemical characteristics. Moreover,the matrix of plant samples is complicated. Thus,quantification of GAs is always extremely difficult. Nowadays,the bottle necks for the study of GAs in plants are due to the lack of efficient sample preparation and sen-sitive detection techniques. This article reviews the analytical methods for determination of GAs in recent years,hoping to provide some references to develop new methods and techniques.