CAJ | 학술논문

目的：建立定量检测血清中胰高血糖素样肽1（GLP-1）酶联免疫吸附试验（ELISA），并探讨其在2型糖尿病（ T2DM）患者个体化治疗中的应用。方法以多克隆抗体作为捕获抗体，另一单克隆抗体作为标记抗体和系列稀释的标准液，建立能够定量检测血清中GLP-1总含量的ELISA，并初步确立生物参考区间。将研究对象根据糖代谢情况分为正常糖耐量（NGT）组（50例）、糖耐量受损（IGT）组（52例）和T2DM组（68例），分别测定空腹、服糖后1 h、服糖后2 h血清中GLP-1含量的动态变化，结合其它常规生化指标，评价GLP-1检测在T2DM患者治疗中的临床应用价值。结果所建立的方法在23．5～1490 nmol／L范围内具有良好的定量关系（R2＝0．999， P＜0．05）；初步建立成人生物参考区间为（79．5±15．6）nmol／L。 T2DM组GLP-1浓度明显高于NGT组和IGT组（P＜0．05）。动态监测研究对象糖耐量试验中血清GLP-1浓度变化，在IGT组和T2DM组出现服糖后GLP-1分泌异常不足的情况。结论成功建立了定量检测血清GLP-1的ELISA，可以动态监测IGT、T2MD患者体内GLP-1的分泌状况以指导临床个体化治疗。
목적：건립정량검측혈청중이고혈당소양태1（GLP-1）매련면역흡부시험（ELISA），병탐토기재2형당뇨병（ T2DM）환자개체화치료중적응용。방법이다극륭항체작위포획항체，령일단극륭항체작위표기항체화계렬희석적표준액，건립능구정량검측혈청중GLP-1총함량적ELISA，병초보학립생물삼고구간。장연구대상근거당대사정황분위정상당내량（NGT）조（50례）、당내량수손（IGT）조（52례）화T2DM조（68례），분별측정공복、복당후1 h、복당후2 h혈청중GLP-1함량적동태변화，결합기타상규생화지표，평개GLP-1검측재T2DM환자치료중적림상응용개치。결과소건립적방법재23．5～1490 nmol／L범위내구유량호적정량관계（R2＝0．999， P＜0．05）；초보건립성인생물삼고구간위（79．5±15．6）nmol／L。 T2DM조GLP-1농도명현고우NGT조화IGT조（P＜0．05）。동태감측연구대상당내량시험중혈청GLP-1농도변화，재IGT조화T2DM조출현복당후GLP-1분비이상불족적정황。결론성공건립료정량검측혈청GLP-1적ELISA，가이동태감측IGT、T2MD환자체내GLP-1적분비상황이지도림상개체화치료。
Objective To establish an enzyme-linked immunosorbent assay ( ELISA ) for the quantitative measuring of glucagon-like peptide 1( GLP-1) in serum and to investigate the application in the individualized treatment of type 2 diabetes mellitus ( T2DM) patients.Methods Using a polyclonal antibody as capture antibody, another monoclonal antibody as labeled antibody, serial dilutions as standard material, an ELISA was established for the quantitative measuring of GLP-1 in serum, and the biological reference interval was also established.All the subjects were classified into 3 groups, normal glucose tolerance (NGT) group (50 cases), impaired glucose tolerance (IGT) group (52 cases) and T2DM group (68 cases).The blood samples were collected at fasting time, 1 h after taking glucose and 2 h after taking glucose.The dynamic change of GLP-1 was evaluated with routine biological items, in order to evaluate the clinical application significance of GLP-1 for the treatment of T2DM.Results The established method had an good quantitative correlation in the range of 23.5-1 490 nmol/L(R2 =0.999, P <0.05).The biological reference interval for adults was (79.5 ±15.6) nmol/L.The GLP-1 in T2DM group was higher than those in NGT and IGT groups (P<0.05).According to the dynamic change of GLP-1 concentration in the oral glucose tolerance test, the deficient secretion of GLP-1 after taking glucose among patients in IGT and T2DM groups could be concluded. Conclusions The ELISA for the quantitative measuring of GLP-1 in serum is successfully established.This method could be used to dynamically monitor the secretion of GLP-1 in IGT and T2DM patients and would be helpful for the individualized treatment of T2DM.